Rabbit Acetylcholine (ACH) ELISA Kit

货号：	AE23409RB
样本：	Serum, Plasma, Other biological fluids
适应物种：	Rabbit (Oryctolagus cuniculus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.78 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：ACh
别名：N/A
检测范围：1.56-100 ng/mL
产品概述：Acetylcholine (Ach) is produced by the synthetic enzyme choline acetyltransferase which uses acetyl coenzyme A and choline as substrates for the formation of acetylcholine.Dietary choline and phosphatidylcholine serve as the sources of free choline for acetylcholine synthesis. Upon release, acetylcholine is metabolized into choline and acetate by acetylcholinesterase, and other nonspecific esterases. Acetylcholine release can be excitatory or inhibitory depending on the type of tissue and the nature of the receptor with which it interacts. Cholinergic receptors can be divided into two types, muscarinic and nicotinic, based on the pharmacological action of various agonists and antagonists. Muscarinic receptors originally were distinguished from nicotinic receptors by the selectivity of the agonists muscarine and nicotine respectively.
特异性：This assay has high sensitivity and excellent specificity for detection of Rabbit ACh. No significant cross-reactivity or interference between Rabbit ACh and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Rabbit ACh and the recovery rates were calculated by comparing the measured value to the expected amount of Rabbit ACh in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rabbit ACh and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate ACh in samples. An antibody specific for ACh has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACh present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACh is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACh bound in the initial step. The color development is stopped and the intensity of the color is measured.