Rabbit Troponin I, slow skeletal muscle (TNNI1) ELISA Kit

货号：	AE13925RB
样本：	Serum, Plasma, Other biological fluids
标记物：	P02645
适应物种：	Rabbit (Oryctolagus cuniculus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	Request Information
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：TNNI1
别名：DKFZp451O223; SSTNI; TNN1; OTTHUMP00000033877|OTTHUMP00000033910|slow-twitch skeletal troponin I|troponin I; skeletal; slow
检测范围：Request Information
产品概述：TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein.TNNI1 is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
特异性：This assay has high sensitivity and excellent specificity for detection of Rabbit TNNI1. No significant cross-reactivity or interference between Rabbit TNNI1 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Rabbit TNNI1 and the recovery rates were calculated by comparing the measured value to the expected amount of Rabbit TNNI1 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rabbit TNNI1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate TNNI1 in samples. An antibody specific for TNNI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.