Rabbit P-Selectin (SELP) ELISA Kit

货号：	AE58161RB
样本：	Serum, Plasma, Other biological fluids
适应物种：	Rabbit (Oryctolagus cuniculus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.60 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：SELP
别名：N/A
检测范围：1.56-100 ng/mL
产品概述：P-selectin is the largest of the known selectins at 140kDa. It contains nine consensus repeats (CR) and extends approximately 40 nm from the endothelial surface. P-selectin is expressed in a-granules of activated platelets and granules of endothelial cells. Within minutes of stimulation of the endothelial cells by inflammatory mediators such as histamine, thrombin, or phorbol esters, P-selectin is surface-expressed. Expression of P-selectin also occurs from the surgical trauma endured during preparation of the tissues for intravital microscopy. The expression is short-lived, reaching its peak after only ten minutes. Additional synthesis of P-selectin is brought about within two hours by cytokines such as interleukin-1 (IL-1) or tumor necrosis factor a (TNF-a).
特异性：This assay has high sensitivity and excellent specificity for detection of Rabbit SELP. No significant cross-reactivity or interference between Rabbit SELP and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Rabbit SELP and the recovery rates were calculated by comparing the measured value to the expected amount of Rabbit SELP in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rabbit SELP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate SELP in samples. An antibody specific for SELP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySELP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELP bound in the initial step. The color development is stopped and the intensity of the color is measured.