Pig Leucine aminopeptidase 3 (LAP3) ELISA Kit

货号：	AE60602PI
样本：	Serum, Plasma, Other biological fluids
标记物：	P28839
适应物种：	Pig (Sus scrofa; Porcine)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.054 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：LAP3
别名：LAP; LAPEP; PEPS; peptidase S
检测范围：0.156-10 ng/mL
产品概述：CXCR4 is an alpha-chemokine receptor specific for stromal-derived-factor-1, a molecule endowed with potent chemotactic activity for lymphocytes. This receptor is one of several chemokine receptors that HIV isolates can use to infect CD4 T cells. Traditionally, HIV isolates that use CXCR4 are known as T-cell tropic isolates. Typically these viruses are found late in infection. It is unclear whether the emergence of CXCR4-using HIV is a consequence or a cause of immunodeficiency. CXCR4 is upregulated during the implantation window in natural and Hormone Replacement Therapy cycles in the endometrium, producing, in presence of a human blastocyst, a surface polarization of the CXCR4 receptors suggesting that this receptor is implicated in the adhesion phase of human implantation.
特异性：This assay has high sensitivity and excellent specificity for detection of Pig LAP3. No significant cross-reactivity or interference between Pig LAP3 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Pig LAP3 and the recovery rates were calculated by comparing the measured value to the expected amount of Pig LAP3 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pig LAP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate LAP3 in samples. An antibody specific for LAP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.