货号: | AE64457PI |
样本: | Serum, Plasma, Other biological fluids |
适应物种: | Pig (Sus scrofa; Porcine) |
应用: | Qualitative |
检测方法: | Competitive ELISA |
检测限: | Request Information |
供应商: | 华夏远洋 |
数量: | 999 |
规格: | 96T/48T |
别名:N/A
检测范围:Request Information
产品概述:
特异性:This assay has high sensitivity and excellent specificity for detection of Pig Parvovirus Antibody. No significant cross-reactivity or interference between Pig Parvovirus Antibody and analogues was observed.
回收率:Matrices listed below were spiked with certain level of recombinant Pig Parvovirus Antibody and the recovery rates were calculated by comparing the measured value to the expected amount of Pig Parvovirus Antibody in samples.
线性:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pig Parvovirus Antibody and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理:This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Parvovirus Antibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Parvovirus Antibody and incubated. The competitive inhibition reaction is launched between with HRP labeled Parvovirus Antibody and unlabeled Parvovirus Antibody with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Parvovirus Antibody in the sample. The color development is stopped and the intensity of the color is measured.