Pig Anti-neutrophil elastase antibody (ANEA) ELISA Kit

货号：	AE62685PI
样本：	Serum, Plasma, Other biological fluids
适应物种：	Pig (Sus scrofa; Porcine)
应用：	Qualitative
检测方法：	Competitive ELISA
检测限：	Request Information
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：ANEA
别名：N/A
检测范围：Request Information
产品概述：Neutrophil elastase (NE) is a causative factor of various inflammatory diseases, including emphysema and rheumatoid arthritis. Induced by endotoxins NE may play a role in the regulation of the antiproteinase (including SLPI and Elafin/SKALP) screen in the lung and the formation of a protective surface at the epithelial site. The antibody labels neutrophils and neutrophil precursors, and also reacts with a minor population of blood monocytes. Neoplastic cells in about 70% of acute myeloid leukaemias are labelled by the antibody, and it is a useful supplement to other antibodies to myeloid markers in the differential identification of acute myeloid leukaemias and extramedullary myeloid cell tumours. Differential identification is aided by the results from a panel of antibodies and traditional histological and enzyme cytochemical methods. Interpretation must be made within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.
特异性：This assay has high sensitivity and excellent specificity for detection of Pig ANEA. No significant cross-reactivity or interference between Pig ANEA and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Pig ANEA and the recovery rates were calculated by comparing the measured value to the expected amount of Pig ANEA in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pig ANEA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANEA. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated ANEA and incubated. The competitive inhibition reaction is launched between with HRP labeled ANEA and unlabeled ANEA with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of ANEA in the sample. The color development is stopped and the intensity of the color is measured.