Chicken Troponin T, fast skeletal muscle (TNNT3) ELISA Kit

货号：	AE13897CH
样本：	Serum, Plasma, Other biological fluids
标记物：	P12620
适应物种：	Chicken (Gallus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	Request Information
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：TNNT3
别名：DKFZp779M2348; TNTF; OTTHUMP00000014435|troponin T; fast skeletal muscle|troponin T3; skeletal; fast|troponin-T3; skeletal; fast
检测范围：Request Information
产品概述：Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B).
特异性：This assay has high sensitivity and excellent specificity for detection of Chicken TNNT3. No significant cross-reactivity or interference between Chicken TNNT3 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Chicken TNNT3 and the recovery rates were calculated by comparing the measured value to the expected amount of Chicken TNNT3 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chicken TNNT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate TNNT3 in samples. An antibody specific for TNNT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.