Bovine Collagen alpha-1 (XVIII) chian (COL18A1) ELISA Kit

货号：	AE63368BO
样本：	Serum, Plasma, Other biological fluids
适应物种：	Bovine (Bos taurus; Cattle)
应用：	Quantitative
检测方法：	Sandwich
检测限：	Request Information
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：COL18A1
别名：FLJ27325; FLJ34914; KNO; KNO1; MGC74745; alpha 1 type XVIII collagen|antiangiogenic agent|endostatin|multi-functional protein MFP
检测范围：Request Information
产品概述：Collagen, type XVIII, alpha 1, also known as COL18A1,alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains.The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Two transcript variants encoding different isoforms have been found for this gene.
特异性：This assay has high sensitivity and excellent specificity for detection of Bovine COL18A1. No significant cross-reactivity or interference between Bovine COL18A1 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Bovine COL18A1 and the recovery rates were calculated by comparing the measured value to the expected amount of Bovine COL18A1 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bovine COL18A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate COL18A1 in samples. An antibody specific for COL18A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCOL18A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL18A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL18A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.