Bovine Lipopolysaccharide binding protein (LBP) ELISA Kit

货号：	AE62951BO
样本：	Serum, Plasma, Other biological fluids
标记物：	Q2TBI0
适应物种：	Bovine (Bos taurus; Cattle)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.288 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：LBP
别名：MGC22233; LPS-binding protein|lipopolysaccharide-binding protein
检测范围：0.625-40 ng/mL
产品概述：LPS-binding protein (LBP) is serum factor known to regulate the endotoxin-induced cellular immune response. Sepsis is a morbid condition induced by a toxin, the introduction or accumulation of which is most commonly caused by infection or trauma. Sepsis-inducing toxins have been found associated with pathogenic bacteria, viruses, plants and venoms.Among the well described bacterial toxins are the endotoxins or lipopolysaccharides (LPS) of the gram-negative bacteria. Upon introduction of LPS into the blood it binds to lipopolysaccharide binding protein (LBP). LBP recognizes the lipid A region of LPS and forms high affinity complexes with both rough and smooth form LPS. During the acute phase, LBP is synthesized by hepatocytes, and reaches very high concentrations in serum.
特异性：This assay has high sensitivity and excellent specificity for detection of Bovine LBP. No significant cross-reactivity or interference between Bovine LBP and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Bovine LBP and the recovery rates were calculated by comparing the measured value to the expected amount of Bovine LBP in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bovine LBP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate LBP in samples. An antibody specific for LBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LBP bound in the initial step. The color development is stopped and the intensity of the color is measured.