Bovine Growth hormone (GH) ELISA Kit

货号：	AE62950BO
样本：	Serum, Plasma, Other biological fluids
标记物：	P01246
适应物种：	Bovine (Bos taurus; Cattle)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.132 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：GH1
别名：GH; GH-N; GHN; IGHD1B; hGH-N; pituitary growth hormone
检测范围：0.312-20 ng/mL
产品概述：Somatotropin is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity.Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus.
特异性：This assay has high sensitivity and excellent specificity for detection of Bovine GH1. No significant cross-reactivity or interference between Bovine GH1 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Bovine GH1 and the recovery rates were calculated by comparing the measured value to the expected amount of Bovine GH1 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bovine GH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate GH1 in samples. An antibody specific for GH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.