Bovine Neuropeptide Y (NPY) ELISA Kit

货号：	AE62949BO
样本：	Serum, Plasma, Other biological fluids
标记物：	Q6RUW3
适应物种：	Bovine (Bos taurus; Cattle)
应用：	Quantitative
检测方法：	Sandwich
检测限：	9.35 pg/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：NPY
别名：PYY4; OTTHUMP00000201946|OTTHUMP00000201947
检测范围：24.69-2000 pg/mL
产品概述：Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter found in the brain and autonomic nervous system.NPY is a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases.
特异性：This assay has high sensitivity and excellent specificity for detection of Bovine NPY. No significant cross-reactivity or interference between Bovine NPY and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Bovine NPY and the recovery rates were calculated by comparing the measured value to the expected amount of Bovine NPY in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bovine NPY and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate NPY in samples. An antibody specific for NPY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNPY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPY bound in the initial step. The color development is stopped and the intensity of the color is measured.