Bovine Lactoperoxidase (LPO) ELISA Kit

货号：	AE62948BO
样本：	Serum, Plasma, Other biological fluids
标记物：	P80025
适应物种：	Bovine (Bos taurus; Cattle)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.055 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：LPO
别名：MGC129990; MGC129991; SPO; salivary peroxidase
检测范围：0.156-10 ng/mL
产品概述：Lactoperoxidase is a peroxidase enzyme found in milk. It is able to form reactive bromine and iodine species, resulting in natural organobromine and organoiodine substances, and can therefore be categorised as a haloperoxidase. It is fairly heat resistant and was widely used as an indicator of overpasteurization of milk.Freshly isolated and purified samples of goat LPO were saturated with ammonium iodide and crystallized using 20% polyethylene glycol 3350 in a hanging drop vapor diffusion setup. Goat LPO consists of a single polypeptide chain of 595 amino acid residues and folds into an oval-shaped structure. The structure contains 20 well-defined alpha-helices of varying lengths including a helix, H(2a), unique to LPO, and two short antiparallel beta-strands.
特异性：This assay has high sensitivity and excellent specificity for detection of Bovine LPO. No significant cross-reactivity or interference between Bovine LPO and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Bovine LPO and the recovery rates were calculated by comparing the measured value to the expected amount of Bovine LPO in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bovine LPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate LPO in samples. An antibody specific for LPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPO bound in the initial step. The color development is stopped and the intensity of the color is measured.