Bovine Anti-Mullerian hormone (AMH) ELISA Kit

货号：	AE62947BO
样本：	Serum, Plasma, Other biological fluids
标记物：	P03972
适应物种：	Bovine (Bos taurus; Cattle)
应用：	Quantitative
检测方法：	Sandwich
检测限：	177.4 pg/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：AMH
别名：MIF; MIS; Mullerian inhibiting factor|Mullerian inhibiting substance
检测范围：493.83-40000 pg/mL
产品概述：Anti-Müllerian hormone (AMH) is a dimeric glycoprotein that inhibits the development of the Müllerian ducts in a male embryo. It is named after Johannes Peter Müller. AMH is a protein hormone structurally related to inhibin and activin, and a member of the transforming growth factor-β (TGF-β) family. It is present in fish, reptiles, birds, marsupials, and placental mammals.In mammals AMH is secreted by Sertoli cells of the testes during embryogenesis of the fetal male and prevents the development of the mullerian ducts into the uterus and other mullerian structures. The effect is ipsilateral, that is each testis suppresses Müllerian development only on its own side. In humans this action takes place by 8 weeks gestation. In female embryogenesis the absence of AMH allows for the development of upper vagina, uterus and cervix, and oviducts.
特异性：This assay has high sensitivity and excellent specificity for detection of Bovine AMH. No significant cross-reactivity or interference between Bovine AMH and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Bovine AMH and the recovery rates were calculated by comparing the measured value to the expected amount of Bovine AMH in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bovine AMH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate AMH in samples. An antibody specific for AMH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAMH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMH bound in the initial step. The color development is stopped and the intensity of the color is measured.