Bovine Galanin (GAL) ELISA Kit

货号：	AE62945BO
样本：	Serum, Plasma, Other biological fluids
标记物：	P11242
适应物种：	Bovine (Bos taurus; Cattle)
应用：	Quantitative
检测方法：	Sandwich
检测限：	Request Information
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：GAL
别名：GALN; GLNN; GMAP; MGC40167; galanin|galanin-message-associated peptide|galanin-related peptide
检测范围：Request Information
产品概述：Galanin is formed by the cleavage of a prepropeptide encoded by a gene known as GAL. It is involved in a number of physiological processes such as regulation of food intake, metabolism and reproduction, regulation of neurotransmitter and hormone release, nociception, intestinal contraction and secretion, and more recently in nervous system development and response to injury. This wide diversity of action is mediated by several galanin receptor subtypes.Galanin is predominantly an inhibitory, hyperpolarizing neuropeptide and as such inhibits neurotransmitter release. Galanin is often co-localized with classical neurotransmitters such as acetylcholine, serotonin and norepinephrine and also with other neuromodulators such as Neuropeptide Y, Substance P and Vasoactive intestinal peptide.
特异性：This assay has high sensitivity and excellent specificity for detection of Bovine GAL. No significant cross-reactivity or interference between Bovine GAL and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Bovine GAL and the recovery rates were calculated by comparing the measured value to the expected amount of Bovine GAL in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bovine GAL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate GAL in samples. An antibody specific for GAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGAL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAL bound in the initial step. The color development is stopped and the intensity of the color is measured.