实时无标记细胞动态分析仪

实时无标记细胞动态分析仪
 

表皮或内皮细胞之间通过紧密连接形成一层具选择性的细胞屏障，细胞屏障不仅控制邻近细胞间间隙对各种溶解物的扩散渗透率，而且调控跨细胞物质转运。细胞屏障的存在一方面保护了机体免受有害物质的伤害，另一方面也限制了治疗性药物的进入。

细胞屏障的通透性可以通过跨膜电阻（即TEER，Transepithelial resistance)来反映，细胞屏障的通透性与跨膜电阻TEER之间的关系为：通透性越高TEER越低，反之亦然。

☆细胞屏障（血脑屏障、鼻黏膜及消化道屏障等）的特性

☆紧密连接动力学

☆新型药物研发

☆药物或毒物对细胞屏障功能的影响

☆肿瘤侵袭转移

☆免疫细胞在中枢神经系统疾病中的作用

1.细胞屏障的特性

 cellZscope可以连续监测细胞几天甚至几星期的生理状态。从cellZscope所提供的跨膜电阻值（TEER）和电容值（Ccl）这两个参数可以获知当前的细胞状态，包括融合度和分化度。CaCO₂是源自人类结肠癌细胞系，通常用于药物转运研究，图中cellZscope跟踪记录了CaCO₂细胞层的形成和分化的信息，自动化的记录了从接种细胞开始并持续观察21天。

2.紧密连接动力学

上皮细胞和内皮细胞的跨膜阻抗值与细胞间紧密连接密切相关。紧密连接可影响细胞间物质转运过程。通过cellZscope测量细胞层跨膜电阻可以用于研究紧密连接动力学。图示MDCK细胞对不同浓度的EGTA的反应。

3.新型药物研发

4.药物或毒物对细胞屏障功能的影响

5.肿瘤侵袭转移

6.免疫细胞在中枢神经系统疾病中的作用

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