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Mouse Complement Component 5 (C5) ELISA Kit

产品价格: ¥1680.00

最小起订量:暂无 可售数量:999盒

发货时限:
暂无
所在地区:
中国上海
有效期至:
长期有效
最后更新:
2021-03-21 01:30:01
浏览次数:
32

产品详情

品牌名称:
加拿大DLDEVELOP
货号: DL-C5-Mu
样本: 血清,血浆,尿液 细胞裂解液,组织匀浆,细胞培养上清液
标记物: 详见说明书
应用: ELISA 定量检测
检测方法: 夹心法
检测限: 详见说明书
供应商: 康朗生物
数量: 大量供应
规格: 96T/盒

Mouse Complement Component 5 (C5) ELISA Kit

Product name: Mouse Complement Component 5 (C5) ELISA Kit
Method: sandwich
Synonyms: CPAMD4; C5a anaphylatoxin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
Catalog number: DL-C5-Mu
Detection range: 0.156-10ng/mL
Size: 96T
Assay length 1-4.5Hours
Price: inquiry
Quality guarantee period: for 12 months

Mouse Complement Component 5 (C5) ELISA Kit

 

Introduction

Item Standard Test Result  
Description This immunoassay kit allows for the specific measurement of this index
in serum, Plasma , Urine ,tissue homogenates and Cell culture supernates and Other biological fluids..
Conform  
Identification Colorimetric Positive  
Composition Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual
1
2
1 × 20ml
1× 120μl
1× 120μl
1 × 12ml
1 × 12ml
1 × 9ml
1 ×6ml
1 ×20ml
2
1
Conform
 

Mouse Complement Component 5 (C5) ELISA Kit

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to this index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for this index and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve

Recovery

Matrices listed below were spiked with certain level of recombinant the index and the recovery humanes were calculated by comparing the measured value to the expected amount of the index in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-93 86
EDTA plasma(n=5) 80-97 88
heparin plasma(n=5) 90-101 95
 

Mouse Complement Component 5 (C5) ELISA Kit

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concenthumanion of the index and their serial dilutions. The results were demonsthumaned by the percentage of calculated concenthumanion to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma(n=5) 88-101% 86-95% 90-102% 80-93%
heparin plasma(n=5) 80-91% 82-90% 95-104% 79-95%
 

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37℃;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
DL-WNT3A-Hu    人无翅型MMTV整合位点家族成员3A(WNT3A) ELISA Kit    human Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit    Homo sapiens human    0.156-10ng/mL    0.062ng/mL    96T    4230    12 months
DL-REG3g-Mu    小鼠再生胰岛衍生蛋白3γ(REG3γ)  ELISA Kit    mouse Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit    Mus musculus  mouse    15.625-1000pg/mL    6.9pg/mL    96T    3980    12 months
DL-RYR1-Mu    小鼠兰尼定受体1(RYR1)  ELISA Kit    mouse Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit    Mus musculus  mouse    0.156-10ng/mL    0.072ng/mL    96T    3980    12 months
DL-NFIB-Mu    小鼠核因子I/B(NFIB)  ELISA Kit    mouse Nuclear Factor I/B (NFIB) ELISA Kit    Mus musculus  mouse    0.156-10ng/mL    0.063ng/mL    96T    3980    12 months
DL-RSPO1-Hu    人R-脊椎蛋白1(RSPO1) ELISA Kit    human R-Spondin 1 (RSPO1) ELISA Kit    Homo sapiens human    15.625-1000pg/mL    5.8pg/mL    96T    4230    12 months

温馨提示:不可用于临床治疗。

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