pGBKT7载体

LOCUS pGBKT7 7303 bp ds-DNA circular SYN 09-九月-2012DEFINITION Yeast two-hybrid ""bait"" vector for expressing proteins fused to the GAL4 DNA-binding domain.ACCESSION .VERSION .KEYWORDS pGBKT7SOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 7303) AUTHORS Clontech TITLE Direct SubmissionCOMMENT This file is created by Vector NTI http://www.biofeng.com/COMMENT VNTAUTHORNAME|biofeng.com|FEATURES Location/Qualifiers source 1..7303 /organism="synthetic DNA construct" /lab_host="Saccharomyces cerevisiae" /mol_type="other DNA" promoter 30..734 /gene="S. cerevisiae ADH1" /label="ADH1 promoter" /label="promoter for?alcohol dehydrogenase 1" CDS 762..1202 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4?(truncated)" /product="DNA binding domain of the GAL4 transcriptional activator" /label="GAL4 DNA binding domain" promoter 1213..1231 /label="T7 promoter" /label="promoter for bacteriophage T7 RNA polymerase" CDS 1245..1247 /codon_start=1 /product="start codon for in vitro transcription/translation" /label="ATG" /translation="M" CDS 1251..1280 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /label="Myc" /translation="EQKLISEEDL" misc_feature 1281..1333 /label="MCS" /label="MCS" /label="multiple cloning site" terminator 1338..1385 /label="T7 terminator" /label="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1412..1599 /gene="S. cerevisiae ADH1" /label="ADH1 terminator" /label="transcription terminator for alcohol dehydrogenase 1" primer_bind complement(1623..1639) /label="M13 rev" /label="common sequencing primer, one of multiple similar variants" protein_bind 1647..1663 /bound_moiety="lac repressor encoded by lacI" /label="lac operator" /label="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1671..1701) /label="lac promoter" /label="promoter for the E. coli lac operon" rep_origin complement(2025..2613) /direction=LEFT /label="ori" /label="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" misc_feature 2942..2989 /label="HSV TK poly(A) signal" /label="herpesvirus thymidine kinase polyadenylation signal" CDS complement(3221..4015) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label="NeoR/KanR" /label="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" promoter complement(4016..4120) /gene="bla" /label="AmpR promoter" rep_origin 4147..5489 /label="2u ori" /label="yeast 2u plasmid origin of replication" promoter 5748..6029 /gene="S. cerevisiae TRP1" /label="TRP1 promoter" CDS 6030..6704 /codon_start=1 /gene="S. cerevisiae TRP1" /product="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" /label="TRP1" /label="yeast auxotrophic marker" rep_origin complement(6807..7262) /direction=LEFT /label="f1 ori" /label="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"ORIGIN 1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg 61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg 121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac 181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga 241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt 301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct 361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac 421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt 601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca 661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa 721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta 781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc 1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcccgggg atccgtcgac 1321 ctgcagcggc cgcataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt 1381 ttttgcgcgc ttgcagccaa gctaattccg ggcgaatttc ttatgattta tgatttttat 1441 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt 1501 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt 1561 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcaagctt ggcgtaatca 1621 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 1681 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 1741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 1801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 1861 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 1921 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 1981 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 2041 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 2101 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 2161 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 2221 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 2281 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 2341 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 2401 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 2461 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 2521 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 2581 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 2641 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 2701 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 2761 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg 2821 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc 2881 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta 2941 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg 3001 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag 3061 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt 3121 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg 3181 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa 3241 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc 3301 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct 3361 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt 3421 ccaccatgat attcggcaag caggcatcgc catgagtcac gacgagatcc tcgccgtcgg 3481 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt 3541 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat 3601 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg 3661 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc 3721 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag 3781 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt 3841 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca 3901 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata 3961 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatactct 4021 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 4081 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 4141 cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta 4201 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg 4261 ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag 4321 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga 4381 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc 4441 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct 4501 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta 4561 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga 4621 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat 4681 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt 4741 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg 4801 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg 4861 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt 4921 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca 4981 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt 5041 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa 5101 aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa 5161 gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact 5221 gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc 5281 atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg 5341 tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca 5401 gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc 5461 aatgctatca tttcctttga tattggatca tactaagaaa ccattattat catgacatta 5521 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt 5581 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 5641 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt 5701 aactatgcgg catcagagca gattgtactg agagtgcacc atagatcaac gacattacta 5761 tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt actttgcagt 5821 tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct tgtcacctta 5881 cgtacaatct tgatccggag cttttctttt tttgccgatt aagaattaat tcggtcgaaa 5941 aaagaaaagg agagggccaa gagggagggc attggtgact attgagcacg tgagtatacg 6001 tgattaagca cacaaaggca gcttggagta tgtctgttat taatttcaca ggtagttctg 6061 gtccattggt gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt gctctagatt 6121 ccgatgctga cttgctgggt attatatgtg tgcccaatag aaagagaaca attgacccgg 6181 ttattgcaag gaaaatttca agtcttgtaa aagcatataa aaatagttca ggcactccga 6241 aatacttggt tggcgtgttt cgtaatcaac ctaaggagga tgttttggct ctggtcaatg 6301 attacggcat tgatatcgtc caactgcatg gagatgagtc gtggcaagaa taccaagagt 6361 tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac atactactca 6421 gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa gcaggtggga 6481 caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa gagagccccg 6541 aaagcttaca ttttatgtta gctggtggac tgacgccaga aaatgttggt gatgcgctta 6601 gattaaatgg cgttattggt gttgatgtaa gcggaggtgt ggagacaaat ggtgtaaaag 6661 actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt actgagtagt 6721 atttatttaa gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata ccgcacagat 6781 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc 6841 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 6901 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 6961 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 7021 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 7081 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 7141 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 7201 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 7261 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gat