pCambia1200载体

LOCUS pCambia1200 9111 bp ds-DNA circular SYN 16-四月-2013DEFINITION Agrobacterium binary vector for plant transformation, with hygromycin- and chloramphenicol-resistance genes.ACCESSION AF234292VERSION .KEYWORDS pCAMBIA1200SOURCE synthetic DNA construct ORGANISM synthetic DNA constructCOMMENT This file is created by Vector NTI http://www.biofeng.com/COMMENT VNTAUTHORNAME|biofeng.com|COMMENT The GenBank record was corrected by inserting a G at position 2281.FEATURES Location/Qualifiers source 1..9111 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" CDS 1219..1848 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label="pVS1 StaA" CDS 2277..3350 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label="pVS1 RepA" /protein_id="" misc_feature 3954..4094 /label="bom" /label="basis of mobility region from pBR322" rep_origin complement(4280..4868) /direction=LEFT /label="ori" /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5139..5798) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label="CmR" /label="confers resistance to chloramphenicol" misc_feature 6326..6350	 /label="LB T-DNA repeat" /label="left border repeat from nopaline C58 T-DNA" polyA_signal 6428..6602 /label="CaMV poly(A) signal" /label="cauliflower mosaic virus polyadenylation signal" CDS complement(6642..7667) /codon_start=1 /product="hygromycin B phosphotransferase" /label="HygR" /label="confers resistance to hygromycin" promoter complement(7735..8412) /label="CaMV 35S promoter (enhanced)" /label="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" promoter 8639..8669 /label="lac promoter" /label="promoter for the E. coli lac operon" protein_bind 8677..8693 /bound_moiety="lac repressor encoded by lacI" /label="lac operator" /label="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8701..8717 /label="M13 rev" /label="common sequencing primer, one of multiple similar variants" misc_feature 8727..8783 /gene=" " /label="MCS" /label="pUC18/19 multiple cloning site" primer_bind complement(8787..8803) /label="M13 fwd" /label="common sequencing primer, one of multiple similar variants" misc_feature 9006..9030 /label="RB T-DNA repeat" /label="right border repeat from nopaline C58 T-DNA"ORIGIN 1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct 61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca 121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt 181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca 241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga 301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca 361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg 421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca 481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg 541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg 601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg 661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga 721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga 781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg 841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac 901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac 961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt 1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg 1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt 1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca 1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc 1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg 1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa 1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc 1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg 1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc 1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa 1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag 1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac 1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc 1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta 1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca 1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc 1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca 2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa 2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc 2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc 2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga 2281 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg 2341 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg 2401 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag 2461 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc 2521 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc 2581 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc 2641 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac 2701 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca 2761 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg 2821 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg 2881 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg 2941 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg 3001 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc 3061 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc 3121 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac 3181 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt 3241 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag 3301 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc 3361 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg 3421 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga 3481 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa 3541 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg 3601 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc 3661 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct 3721 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc 3781 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt 3841 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 3901 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 3961 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 4021 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 4081 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 4141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 4201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 4261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 4321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 4381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 4441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 4501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 4561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 4621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 4681 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 4741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 4801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 4861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 4921 cacgttaagg gattttggtc atgcatgata tatctcccaa tttgtgtagg gcttattatg 4981 cacgcttaaa aataataaaa gcagacttga cctgatagtt tggctgtgag caattatgtg 5041 cttagtgcat ctaatcgctt gagttaacgc cggcgaagcg gcgtcggctt gaacgaattt 5101 ctagctagag gatcgcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca 5161 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca 5221 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 5281 attgtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 5341 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa 5401 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 5461 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg 5521 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg 5581 aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg 5641 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta 5701 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat 5761 atatcaacgg tggtatatcc agtgattttt ttctccatga tgtttaactt tgttttaggg 5821 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg 5881 taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acatgtcata 5941 acaagaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct 6001 ggaccagttg cgtgacggca gttacgctac ttgcattaca gcttacgaac cgaacgaggc 6061 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac 6121 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg 6181 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc 6241 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc 6301 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc 6361 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa 6421 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat 6481 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc 6541 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga 6601 aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt tgccctcgga 6661 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag 6721 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg 6781 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc 6841 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg cgatcctgca 6901 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc 6961 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag 7021 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg 7081 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc 7141 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga 7201 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg 7261 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catagcctcc 7321 gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc 7381 tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat gtcaagcact 7441 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa 7501 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa 7561 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg 7621 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc 7681 ccgggatctg cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg 7741 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga aggatagtgg 7801 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac 7861 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga 7921 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg 7981 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg 8041 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct 8101 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgtta 8161 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc 8221 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc 8281 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg 8341 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt 8401 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag 8461 acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc 8521 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 8581 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 8641 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 8701 caggaaacag ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctctagag 8761 tcgacctgca ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa 8821 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 8881 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 8941 tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca 9001 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac 9061 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt g