Anti-Vasoactive Intestinal Peptide (human, bovine, porcine, rat, VIP)

Cat. No.

ABS 023-02

Product name

Anti-Vasoactive Intestinal Peptide (human, bovine, porcine, rat, VIP)

Description

Mouse monoclonal antibody

Specificity

ABS 023-02 binds to human, bovine, porcine or rat VIP, but does not bind to PHM or PACAP

Immunogen

Vasoactive intestinal peptide 

Application

ELISA, WB

Species reactivity (positive)

Human, bovine, porcine, rat

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Gene ID

7432

Clone number

2

Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Vasoactive intestinal peptide (VIP) is a 28 amino acid peptide belonging to the secretin-glucagon family of peptide hormones. Its sequence is identical in humans, cows, pigs and rats. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome.
VIP shows high sequence homology with peptide histidine-methionine (PHM) in humans or peptide histidine-serine (PHI) in pigs or rats, present in the same precursor, and with the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP).

Application 1

ELISA
A dilution guideline of 1/1000 applies to VIP coated directly at 2 µg/mL. For glutaraldehyde-linked VIP-ovalbumin (molar ratio 4:1) coated at 2 µg/mL, the dilution is 1/32,000.

Application 2

WB
ABS 023-02 can be used in Western blotting.

Application 3

IHC
ABS 023-02 has been used in IHC.

Formalin fixed paraffin-embedded human appendix tissue stained with ABS 023-02. Dilution: 1:100

Formalin fixed paraffin-embedded human appendix tissue stained with ABS 023-02. Dilution: 1:100

Formalin fixed paraffin-embedded human pancreas tissue stained with ABS 023-02. Dilution: 1:100

 

Application 4

IF 

ABS 023-02 has been used in IF.

IF staining in human pancreas tissue stained with ABS 023-02. Signal is strong in nerves of exocrine part of pancreas. Dilution: 1:100.