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人OP酶联免疫检测试剂| 货号： | HYB 314-011 |
| 抗体名： | Anti-Gliadin peptide |
| 抗体英文名： | Anti-Gliadin peptide |
| 靶点： | 见官方网站 |
| 浓度： | 1 mg/ml |
| 应用范围： | ELISA, WB |
| 宿主： | Wheat, Rye, Barley |
| 供应商： | 上海泽叶生物科技有限公司 |
| 数量： | 30 |
| 级别： | 见官方网站 |
| 目录编号： | HYB 314-011 |
| 抗原来源： | 见官方网站 |
| 保质期： | 1年 |
| 适应物种： | 见官方网站 |
| 标记物： | 见官方网站 |
| 克隆性： | 否 |
| 保存条件： | -20ºC |
| 形态： | 冻干粉或液体 |
| 亚型： | 见官方网站 |
| 免疫原： | 见官方网站 |
| 规格： | 200ug |
Anti-Gliadin peptide
Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站：www.bioporto.com
- Cat. No.
- HYB 314-011
- Product name
- Anti-Gliadin peptide
- Description
-
Mouse monoclonal antibody
- Specificity
-
HYB 314-01 reacts strongly with the deamidated peptide but cross-reacts about 5% with the non-deamidated peptide KLQPFPQPQLPYPQPQ.
- Immunogen
-
Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ)
- Application
- ELISA, WB
- Species reactivity (positive)
- Wheat, Rye, Barley
- Species reactivity (negative)
- Oat
- Subclass
-
IgG2a/k
- Clone number
- 14D5
- Epitope
-
The epitope is located in a central region of the Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) peptide (1).
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
- Application 1
-
ELISA
HYB 314-01 reacts with both the deamidated peptide (KLQPFPQPELPYPQPQ) and non-deamidated petide (KLQPFPQPQLPYPQPQ) when these are coated directly in an immunoplate, but approx. 20-fold higher concentrations of antibody are needed to produce the same signal with the non-deamidated peptide. (2) - Application 2
-
WB
HYB 314-01 can be used in western blotting.
A western blot of Gliadin peptide (HYB 314-01) measured in rye (lane 1), barley (lane 2) and wheat (lane 3). The SDS gel was run under reducing conditions, and HYB 314-01 diluted 1:1000.
