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SBA(SouthernBiotech)05-8340-09Mouse
多壁碳纳米管
间苯二胺盐酸盐, AR,>99.0%(T)
通过认证 
Aclidinium Bromide
FH1(BRD-K4477)
MK-801 (Dizocilpine)
Venetoclax (ABT-199,
TSU-68 (SU6668, Oran
Sapogenins Glycoside| 货号： | S1459 |
| 规格： | 5mg/10mM/1mL/10mg/50mg |
更多详情请访问中国唯一官方网站： http://www.selleck.cn/products/Thiazovivin.html
selleck产品在文献中的引用:
| Cell Reprogram, 2012, 14(6):485-96 |
| Cell Res, 2013, 23(10):1187-200 |
| Arch Toxicol, 2013, 87(12):2215-31 |
客户使用selleck产品的实验数据:
生物活性
| 产品描述 | Thiazovivin是一种新型ROCK抑制剂，IC50为0.5 μM，在单细胞分离后，促进人胚胎干细胞（hESC）的存活。 | |||||
|---|---|---|---|---|---|---|
| 靶点 | ROCK | |||||
| IC50 | ~0.5 μM [1] | |||||
| 体外研究 | Although displaying little impact on cell proliferation, Thiazovivin treatment significantly enhances the survival of human embryonic stem cells (hESCs) after enzymatic dissociation more than 30-fold, while homogenously maintaining pluripotency with the characteristic colony morphology, expression of typical pluripotency markers such as alkaline phosphatase (ALP), and normal karyotype. Dissociated hESCs treated with Thiazovivin display dramatically increased adhesion to matrigel- or laminin-coated plates but not to gelatin-coated plates within a few hours. Thiazovivin treatment increases cell-ECM adhesion-mediated β1 integrin activity, which synergizes with growth factors to promote cell survival. In addition to activating integrin, Thiazovivin but not Tyrintegin (Ptn) protects hESCs from death in the absence of ECM in suspension through E-cadherin-mediated cell-cell interaction. Thiazovivin treatment potently inhibits endocytosis of E-cadherin, consequently stabilizing E-cadherin on the cell surface and leading to reestablishment of cell-cell interaction, which is essential for hESC survival in ECM-free conditions. Thiazovivin but not Tyrintegin (Ptn) at 2 μM inhibits Rho-associated kinase (ROCK) activity and protects hESCs at a similar level as the widely used selective ROCK inhibitor Y-27632 at 10 μM, suggesting that Rho-ROCK signaling regulates cell-ECM and cell-cell adhesion. [1] Thiazovivin at 1 μM increases the reprogramming efficiency of CB mononuclear cells to induced pluripotent stem cells (iPSCs) by more than 10 times. [2] | |||||
| 体内研究 | ||||||
| 特征 | ||||||
推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)
激酶实验:
[1]
| In vitro ROCK assay | Thiazovivin is dissolved in DMSO. CycLex Rho-kinase assay kit is used to detect ROCK activity using recombinant ROCK in the presence of increasing concentrations of Thiazovivin (~10 μM). |
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参考文献
[1] Xu Y, et al. Proc Natl Acad Sci U S A, 2010, 107(18), 8129-8134.
[2] Hu K, et al. Blood, 2011, 117(14), e109-119.
[3] Lin T, et al. Nat Methods, 2009, 6(11), 805-808.
温馨提示：不可用于临床治疗。
