货号: | D5005 |
供应商: | 科昊佳 |
数量: | 现货 |
英文名: | EZ DNA Methylation-Gold™ Kit |
规格: | 50 Rxns |
Zymo于1994年成立于美国加州Orange County,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒,其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各顶级实验室的认可,产品具有“快速、简便、超纯”的特点;
Zymo甲基化产品线已被上千次引用在各大主流学术刊物上,包括NATURE、SCIENCE 、PNAS、Stem Cells、 Nucleic Acids Res等;迄今,EZ DNA Methylation™系列产品作为DNA重亚硫酸盐处理(DNA甲基化分析前处理)的的金标准,已成为最畅销和引用次数最多的产品;
产品 | 规格 | 货号 | 报价 |
EZ DNA Methylation-Gold™ Kit | 50 Rxns | D5005 | 2117 |
EZ DNA Methylation-Gold™ Kit | 200 Rxns | D5006 | 7109 |
- 富含GC的DNA亚硫酸盐转化过程少于3小时
- 热变性/转化反应简化了非甲基化的胞嘧啶转化为尿嘧啶的过程
- 采用离心柱对硫酸盐处理的DNA进行脱硫化(desulphonation)和回收
- 回收的DNA可用于PCR、内切酶消化、测序、芯片分析等
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产品介绍The EZ DNA Methylation-Gold™ Kit is a refinement of our popular EZ DNA Methylation™ kits. These products consolidate DNA denaturation and bisulfite conversion processes into one step, leading to a much faster bisulfite conversion. This is accomplished using temperature denaturation to complement chemical denaturation in the previous protocol. Also, this kit have been streamlined for high yield recovery of DNA following bisulfite treatment. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. 产品特点 形式: Spin Column DNA回收率: > 75% 实验时间: 3 hrs 设备: Microcentrifuge 结合能力: 5 µg/prep 洗脱体积: ≥ 10 µl 转化率: > 99% 样品: Plasmid, genomic, and endonuclease digested DNAs.