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Anti-phospho-IR/IGF1R (Tyr1158/Tyr1
Proteintech现货SMARCAL1蛋白货号:Ag2
CD193 (CCR3)
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IV型胶原蛋白/4型胶原
MicroVue CICP EIA*| 货号： | C-750 |
| 抗体名： | Cyclopiazonic Acid |
| 抗体英文名： | Cyclopiazonic Acid |
| 靶点： | 见官方网站 |
| 浓度： | 见官方网站 |
| 应用范围： | 见官方网站 |
| 宿主： | Natural |
| 供应商： | 上海信裕生物科技有限公司 |
| 数量： | 大量 |
| 级别： | 高 |
| 目录编号： | C-750 |
| 抗原来源： | Natural |
| 保质期： | 6个月 |
| 适应物种： | 见官方网站 |
| 标记物： | 见官方网站 |
| 克隆性： | 否 |
| 保存条件： | -20°C |
| 形态： | 液体或冻干粉 |
| 亚型： | 见官方网站 |
| 免疫原： | 见官方网站 |
| 规格： | 5 mg, 10 mg, 25 mg, 50 mg |
Cyclopiazonic Acid
CPAA Potent Inhibitor of Intracellular Ca2+-ATPase Pump
Cat #: C-750
Sizes: 5 mg, 10 mg, 25 mg, 50 mg
Source: Natural
MW: 336.4 Da.
Target: SERCA pump
CLICK HERE TO RECEIVE A 1 mg FREE TRIAL SAMPLE!
Cyclopiazonic acid (CPA) is a toxic fungal secondary metabolite. Chemically, it is an indole tetrameric acid. CPA was originally isolated from Penicillium cyclopium and subsequently from other P. cyclopium, Penicillium griseofulvum, Penicillium camembertii, Aspergillus flavus and Aspergillus versicolor.
CPA is a highly potent, selective and reversible inhibitor of the sarcoplasmic reticulum (ER) Ca2+ – ATPase pump (SERCA). This Ca2+ pump is an integral membrane protein of 994 amino acids that consists of three cytoplasmic domains containing the ATP binding site and ten transmembrane helices containing the two Ca2+binding sites.1,2
The interchange of Ca2+ between the cell cytosol and the ER lumen by SERCA is a central process in intracellular signaling. Moreover, this process has a critical role at the muscle contraction-relaxation cycle, in which, the recovery phase relies on transport of calcium into the ER by the action of the SERCA. Inhibition of this pump leads to decrease of Ca2+ uptake from the cytosol to the ER Lumen, thereby depletion of Ca2+ store operated and increase of cytosolic Ca2+. 3-4
CPA inhibits the Ca2+ pump by blocking the Ca2+ access channel and immobilizing a subset of transmembrane helices.5
Alomone Labs is pleased to offer Cyclopiazonic Acid (#C-750) a natural toxin isolated from the Penicillium griseofulvum and purified to homogeneity.
References
- Suzuki, M. et al. (1992 ) Br J Pharmacol. 107, 134.
- Demaurex, N. et al. (1992) J. Biol. Chem. 267, 2318.
- Zhang, P. et al. (1998) Nature 392, 835.
- Xu, C. et al. (2002) J. Mol. Biol. 316, 201.
- Moncoq, K. et al. (2007) J. Biol. Chem. 282, 9748.
For a list of product citations in the literature, see product citations tab below. If you know of additional relevant citations for this product, please let us know.
- Our Bioassay
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Alomone Labs Cyclopiazonic Acid increases cytosolic Ca2+ in HL60 cells.
Intracellular Ca2+ levels from Fluo-3 AM loaded cells stimulated with increasing concentrations of Cyclopiazonic Acid (#C-750) in the presence of 4 mM EGTA.
OriginIsolated from Penicillium griseofulvum.
Purity>98%.
Effective concentration1-10 µM.
Chemical name(6aR,11aS,11bR)-rel-10-Acetyl-2,6,6a,7,11a,11b,hexahydro-7,7-dimethyl-9H-pyrrolo[1',2':2,3]isoindolo[4,5,6-cd] indol-9-one.
Molecular formulaC20H20N2O3.
CAS number18172-33-3.
Activity
Cyclopiazonic Acid is a cell-permeable, reversible inhibitor of sarcoplasmic reticulum Ca2+-ATPase1,2.
Storage before reconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
ReconstitutionDMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitutionUp to one week at 4°C or three months at -20°C.
Product citations
- Forostyak, O. et al. (2016) Stem Cell Res. 16, 622.
- Wang, Y. et al. (2015) Am. J. Physiol. Renal. Physiol. 308, F1135.
- Chaudhari, S. et al. (2014) Am. J. Physiol. 306, F1069.
- Grigoryan, G. and Segal, M. (2013) J. Neurophysiol. 110, 279.
- Perez, G.J. et al. (2013) Am. J. Physiol. 304, C280.
- Grigoryan, G. et al. (2012) Hippocampus 22, 1635.
- Komori, Y. et al. (2010) Cell Calcium 48, 324.
- Malkinson, G. and Spira, M.E. (2010) Cell Calcium 47, 315.
- Hegg, C.C. et al. (2009) Glia 57, 634.
- Kim, M.S. et al. (2009) J. Biol. Chem. 284, 9733.
