点击图片查看原图

pLVX-EF1伪-IRES-ZsGreen1载体

产品价格: ¥1500.00

最小起订量:暂无 可售数量:999盒

发货时限:
暂无
所在地区:
中国上海
有效期至:
长期有效
最后更新:
2021-05-22 01:30:02
浏览次数:
59

产品详情

品牌名称:
盖宁生物
货号: GN2014
供应商: 上海盖宁生物
数量: 现货
英文名: pLVX-EF1伪-IRES-ZsGreen1
保质期: 三年
保存条件: 常温
规格: 5ug质粒
质粒类型: 慢病毒载体
高拷贝/低拷贝: 高拷贝
启动子: EF1α/ EF1a
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8889 bp 
5' 测序引物及序列: EF1a Forward: TCAAGCCTCAGACAGTGGTTC
3' 测序引物及序列: IRES-R: CCTCACATTGCCAAAAGACG
载体抗性: Ampicillin (氨苄青霉素)
备注: 载体能够表达ZsGreen1荧光蛋白

订购信息

产品编号 产品名称 规格 价格
2014 pLVX-EF1α-IRES-ZsGreen1

5ug质粒

¥1500.00

质粒图谱

pLVX-EF1α-IRES-ZsGreen1 质粒图谱

载体描述

pLVX-EF1α-IRES-ZsGreen1 is an HIV-1-based, lentiviral expression vector designed to simultaneously and constitutively express a protein of interest and the green fluorescent protein ZsGreen1 from a bicistronic transcript in mammalian cells. ZsGreen1 is a human-codon-optimized variant of the reef coral Zoanthus sp. green fluorescent protein (ZsGreen) that has been engineered for brighter fluorescence (1–3). The excitation and emission maxima of native ZsGreen1 are 493 nm and 505 nm, respectively.

Simultaneous expression of a protein of interest and ZsGreen1 is made possible by the presence of an encephalomyo- carditis virus internal ribosome entry site (IRES; 4, 5) positioned between the multiple cloning site (MCS) and the ZsGreen1 gene. The IRES allows a protein of interest and ZsGreen1 to be translated from a single bicistronic mRNA. Stable, constitutive expression of the bicistronic transcript is driven by the EF1α promoter (PEF1α), which continues to be constitutively active even after vector integration into the host cell genome (6).

pLVX-EF1α-IRES-ZsGreen1 contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral RNA (7), leading to increased viral titers from packaging cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (9). Finally, pLVX-EF1α-IRES-ZsGreen1 also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, this element creates a central DNA flap that increases nuclear import of the viral genome, resulting in improved vector integration and more efficient transduction (9). The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.

温馨提示:不可用于临床治疗。

免责声明

本网页所展示的有关【pLVX-EF1伪-IRES-ZsGreen1载体】的信息/图片/参数等由的会员【上海盖宁生物科技有限公司 】提供,由【生物实验采购网】会员【上海盖宁生物科技有限公司 】自行对信息/图片/参数等的真实性、准确性和合法性负责,本平台(本网站)仅提供展示服务,请谨慎交易,因交易而产生的法 律关系及法律纠纷由您自行协商解决,本平台(本网站)对此不承担任何责任。您在本网页可以浏览【pLVX-EF1伪-IRES-ZsGreen1载体】有关的信息/图片/价格等及提供 【pLVX-EF1伪-IRES-ZsGreen1载体】的商家公司简介、联系方式等信息。

在您的合法权益受到侵害时,请您致电,我们将竭诚为您服务,感谢您对【生物实验采购网】的关注与支持!

网站首页 | 关于我们 | 联系方式 | 使用协议 | 版权隐私 | 网站地图  |  排名推广  |  广告服务  |  积分换礼  |  网站留言  |  RSS订阅  |  违规举报
 
免责声明:本站有部分内容来自互联网,如无意中侵犯了某个媒体 、公司 、企业或个人等的知识产权,请来电或致函告之,本网站将在规定时间内给予删除等相关处理。