货号：	KL-EL001940HU
样本：	血清/组织/尿液
标记物：	HRP标记物
应用：	科研
检测方法：	酶联免疫
检测限：	见说明书
供应商：	康朗生物
数量：	1000

Human Apolipoprotein L1(APOL1) ELISA kit

PRINCIPLE OF THE ASSAY 
This assay employs the competitive inhibition enzyme immunoassay technique. 
The microtiter plate provided in this kit has been pre-coated with APOL1. 
Standards or samples are added to the appropriate microtiter plate wells with 
Horseradish Peroxidase (HRP) conjugated antibody preparation specific for 
APOL1. The competitive inhibition reaction is launched between with pre-coated 
APOL1 and APOL1 in samples. A substrate solution is added to the wells and 
the color develops in opposite to the amount of APOL1 in the samples. The color 
development is stopped and the intensity of the color is measured. 

Human Apolipoprotein L1(APOL1) ELISA kit

DETECTION RANGE 
7.81 ng/ml-500 ng/ml. 

Human Apolipoprotein L1(APOL1) ELISA kit

SENSITIVITY 
The minimum detectable dose of human APOL1 is typically less than 1.95 ng/ml. 
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as 
the lowest protein concentration that could be differentiated from zero. It was 
determined the mean O.D value of 20 replicates of the zero standard added by 
their three standard deviations. 

Human Apolipoprotein L1(APOL1) ELISA kit

SPECIFICITY 
This assay has high sensitivity and excellent specificity for detection of 
human APOL1. No significant cross-reactivity or interference between human 
APOL1 and analogues was observed. 
Note: Limited by current skills and knowledge, it is impossible for us to complete 
the cross-reactivity detection between human APOL1 and all the analogues, 
therefore, cross reaction may still exist. 
  
3 
PRECISION 
Intra-assay Precision (Precision within an assay): CV%<8% 
Three samples of known concentration were tested twenty times on one plate to 
assess. 
Inter-assay Precision (Precision between assays): CV%<10% 
Three samples of known concentration were tested in twenty assays to assess. 
LIMITATIONS OF THE PROCEDURE 
 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC 
PROCEDURES. 
 The kit should not be used beyond the expiration date on the kit label. 
 Do not mix or substitute reagents with those from other lots or sources. 
 If samples generate values higher than the highest standard, dilute the 
samples with Sample Diluent and repeat the assay. 
 Any variation in Sample Diluent, operator, pipetting technique, washing 
technique, incubation time or temperature, and kit age can cause variation 
in binding. 
 This assay is designed to eliminate interference by soluble receptors, 
binding proteins, and other factors present in biological samples. Until all 
factors have been tested in the Immunoassay, the possibility of 
interference cannot be excluded.