Human Breast cancer susceptibility protein 1 (BRCA1) ELISA Kit

货号：	AE63118HU
样本：	Serum, Plasma, Other biological fluids
标记物：	P38398
适应物种：	Human (Homo sapiens)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.065 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：BRCA1
别名：BRCAI; BRCC1; BROVCA1; IRIS; PSCP; RNF53; BRCA1/BRCA2-containing complex; subunit 1|RING finger protein 53|breast and ovarian cancer susceptibility protein 1|breast and ovarian cancer sususceptibili
检测范围：0.156-10 ng/mL
产品概述：BRCA1  is a human gene, some mutations of which are associated with a significant increase in the risk of breast cancer, as well as other cancers. BRCA1 belongs to a class of genes known as tumor suppressors, which maintains genomic integrity to prevent dangerous genetic changes. The multifactorial BRCA1 protein product is involved in DNA damage repair especially error-free repair of DNA double strand breaks, ubiquitination, transcriptional regulation as well as other functions.The BRCA1 protein is directly involved in the repair of damaged DNA. In the nucleus of many types of normal cells, the BRCA1 protein is thought to interact indirectly with RAD51 during repair of DNA double-strand breaks, though the details and significance of this interaction is the subject of debate.
特异性：This assay has high sensitivity and excellent specificity for detection of Human BRCA1. No significant cross-reactivity or interference between Human BRCA1 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Human BRCA1 and the recovery rates were calculated by comparing the measured value to the expected amount of Human BRCA1 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human BRCA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate BRCA1 in samples. An antibody specific for BRCA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBRCA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BRCA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BRCA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.