货号: | E1012 |
供应商: | 无锡柏欧美地生物 |
保质期: | 12个月 |
数量: | 大量 |
保存条件: | 4ºC |
规格: | 200 µg/ml |
甘油(组织细胞)酶法测定试剂盒(Tissue glycerol assay kit, E1012) |
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甘油(组织细胞)酶法测定试剂盒(Tissue glycerol assay kit, E1012) E1012 105 assays 780元 价格说明:市场的9折,量多优惠,欢迎洽谈! 描述:甘油是甘油三酯的水解产物。与游离脂肪酸一样,甘油含量是甘油三酯水解反应的可靠检测指标,但检测更加方便。试剂盒采用优化步骤,检测实体组织、细胞中甘油含量。 原理:在ATP存在下甘油被甘油激酶磷酸化为3-磷酸甘油,再被甘油磷酸氧化酶氧化产生过氧化氢;在过氧化物酶作用下生色底物转化为苯醌亚胺,光密度值与甘油浓度成正比。 用途:本kit测定实体组织、细胞中的甘油浓度。测定血液、培养基、体液、饮品中甘油使用E1002。 组成 (105次微板测定或30次1 ml比色杯测定) (1) 裂解液 100 ml (2) R1试剂 16 ml (3) R2试剂 4 ml (4) 4 mmol/L甘油标准品1 ml 4 ºC避光储存3月。 所需设备:酶标仪、生化分析仪或721、722型可见光分光光度计。96孔微板或比色杯光径1cm。最佳工作波长550nm,如无此波长建议优先选用570nm、次选530、490nm。 注:此产品曾使用过E1002-2货号,系同一种产品。 部分发表论文: Xu Chong, He Jinhan, Jiang Hongfeng, Zu Luxia, Zhai Wenjie, Pu Shenshen, Xu Guoheng, 2009. Direct Effect of Glucocorticoids on Lipolysis in Adipocytes. Molecular Endocrinology, 23(8): 1161-1170. IF: 4.888 He Yonghan, Zhang Huaqi, Teng Jianghua, Huang Lina, Li Ying, Sun Changhao, 2011. Involvement of calcium-sensing receptor in inhibition of lipolysis through intracellular cAMP and calcium pathways in human adipocytes. Biochemical and Biophysical Research Communications, 404(1): 393-399. IF: 2.593 Shan T., Ren Y., Liu Y., Zhu L., Wang Y., 2010. Breed difference and regulation of the porcine Sirtuin 1 by insulin. American Society of Animal Science, 88: 3909-3917. IF: 2.58 Zhai W., Xu C., Ling Y., Liu S., Deng J., Qi Y., Londos C., Xu G., 2010. Increased Lipolysis in Adipose Tissues is Associated with Elevation of Systemic Free Fatty Acids and Insulin Resistance in Perilipin Null Mice. Hormone Metabolic Research, 42(4): 247-253. IF: 2.414 Shan T., Wu T., Reng Y., Wang Y., 2009. Breed difference and regulation of the porcine adipose triglyceride lipase and hormone sensitive lipase by TNFα. Animal Genetics, 40(6): 863-870. IF: 2.203 Liang Jing, Zhang Xiaowei, Zhao Ruqian, Maak Steffen, Yang Xiaojing, 2011. Effect of maternal protein restriction on lipid metabolism in Meishan piglets at weaning. Livestock Science, 136: 157-163. IF: 1.295 |