货号: | PHY0029A |
抗体名: | GLN1/2 / Anti-GLN1/2 glutamine synthetase antibody |
浓度: | 150ug |
应用范围: | WB |
宿主: | 兔 |
适应物种: | Arabidopsis thaliana |
克隆性: | 否 |
形态: | 冻干粉 |
Product Information
Description: | Rabbit polyclonal antibody |
Background: | Glutamine synthetase catalyses the synthesis of glutamine from glutamate and ammonia, which is one of the key enzymes involved in nitrogen metabolism of plants. Two classes of glutamine synthetase are present in plants, cytosolic form (GLN1) and chloroplastic form (GLN2). While GLN1 is encoded by five genes (AT5G37600, AT1G66200, AT3G17820, AT5G16570, AT1G48470), GLN2 is encoded by a single gene (AT5G35630). |
Synonyms: | GLN1/2. |
Immunogen: | KLH-conjugated synthetic peptide of GLN1 derived from Arabidopsis thaliana AT5G37600, AT1G66200, AT3G17820, AT1G48470. |
Form: | Lyophilized |
Quantity: | 150 µg |
Puirification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150 µl of 0.01 M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 39-40 kDa (GLN1,cytoplasmic form), 44-45 kDa (GLN2, chloroplastic form) |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Solanum lycopersicum, Spinacia oleracea, Zea mays, Oryza sativa Japonica Group, Nicotiana tabacum, Vitis vinifera, Brassica napus, Populus trichocarpa, Solanum tuberosum, and 80-99% homologues with the sequence in Triticum aestivum, Cucumis sativus, Gossypium raimondii, Hordeum vulgare. |