货号: | Z01003 |
保存条件: | -20 |
供应商: | 上海闪晶生物 |
肠激酶(Enterokinase) 本产品为基因重组的高活性高纯度牛肠激酶轻链,是一种切割位点高度专一的蛋白酶。识别序列DDDDK,在赖氨酸残基的C 端切割多肽。该酶可将带有识别序列的融合蛋白切开。肠激酶在很多反应条件下仍保持活性,可以在较宽pH 范围(4.5-9.5),较宽温度范围(4-45℃)以及多种去污剂和变性剂存在条件下有效切割融合蛋白。 纯度:经SDS-PAGE 电泳分析,大于95%。 活性单位:一个活性单位定义为在22℃条件下,8 小时将 50μg 胰蛋白酶原95%转化为胰蛋白酶所需的酶量。 浓度:2U/ul 价格:500.00元/100U 酶切条件:融合蛋白 1~1.5mg/ml (100μl) 重组肠激酶 2~3U 10×缓冲液 10μl 温度(℃) 22℃ 时间(小时) 8~16 保存条件:-20℃保存 注意: 在含有过量的Urea(>2M),NaCl(>250mM),ß-BE(>20mM),SDS(>0.1%),imidazole(>50mM)等,以及在PH>-或PH<6的缓冲体系条件下酶切,都将影响Enterokinase的正常活性. Recombinant Enterokinase Description Recombinant enterokinase (rEK) is the catalytic subunit of bovine enterokinase, which is and purified to yield a high enzyme activity preparation. rEK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this sequence. Unit Definition: One unit of rEK is the amount of enzyme required to digest 50ug of thioredoxin-NP-27 fusion protein to 95% completion in 8 hours at 22℃. Storage: rEK in 50 mM potassium phosphate, pH 8.0, 500 mM NaCl and 50% glycerol should be stored at -20°C. Guaranteed stable for 1 year when stored properly. 10x Reaction Buffer: 500 mM Tris-HCl, pH 8.0 (22°C), 10 mM CaCl2, 1% Tween-20 |