货号: | 740412.50 |
规格: | 50次 |
特点:
- 节约60%的时间,增加100%的产量
- 新一代阴离子交换-超快,高产量的转染级别的质粒DNA
- 优化设计的柱子:制备时间约为30 分钟(Midi prep);35 分钟(Maxi prep)
- 包含柱过滤器:高过虑流速,清除裂解液中不溶物的同时可向柱子加样
- 改进的硅材料:标准的产量250μg-1000μg
- 专利的阴离子交换技术得到转染级别的质粒DNA
- NucleoBond(R)Xtra Plus kits 包含NucleoBond(R) Finalizers;加速了DNA 的沉淀
产品概览
NucleoBond(R) XtraMidi | NucleoBond(R) Xtra Maxi | |||
技术 | 阴离子交换色谱法 | |||
产品形式 | Midi gravity-flow columns | Maxi gravity-flow columns | ||
裂解液澄清 | 柱过滤器 | 柱过滤器 | ||
样品材料 | < 200 mL (高拷贝) < 400 mL (低拷贝) | < 600 mL (高拷贝) < 1 200 mL (低拷贝) | ||
载体大小 | < 300 kbp | < 300 kbp | ||
产量 | 250 μg | 1 000 μg | ||
A260/A280 | 1.80 – 1.95 | 1.80 – 1.95 | ||
异丙醇沉淀 | Xtra Midi | Xtra Midi Plus | XtraMaxi | XtraMaxi Plus |
Centrifugation | NucleoBond(R) Finalizer | Centrifugation | NucleoBond(R)Finalizer Large | |
制备时间 | 70min/4preps | 30min/4preps | 80min/4preps | 35min/4preps |
应用:
从大肠杆菌中提取高拷贝/低拷贝质粒
订购信息
产品 | 规格 | 备注 | 货号 |
NucleoBond® Xtra Midi kits | |||
NucleoBond® Xtra Midi | 10 | NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters,buffers, RNase A | 740410.10 |
50 | 同上 | 740410.50 | |
100 | 同上 | 740410.100 | |
NucleoBond® Xtra Midi Plus | 10 | NucleoBond® Xtra Midi with inserted NucleoBond® Columns Xtra Column Filters,NucleoBond® Finalizers, buffers, RNase A | 740412.10 |
50 | 同上 | 740412.50 | |
NucleoBond® Xtra Maxi kits | |||
NucleoBond® Xtra Maxi | 10 | NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A | 740414.10 |
50 | 同上 | 740414.50 | |
100 | 同上 | 740414.100 | |
NucleoBond® Xtra Maxi Plus | 10 | NucleoBond® Xtra Maxi with inserted NucleoBond® Xtra Column Filters, Columns NucleoBond® Finalizers Large, buffers, RNase A | 740416.10 |
50 | 同上 | 740416.50 | |
产品配件 | 包装 | 备注 | 货号 |
NucleoBond® Xtra Combi Rack | 1 | for use with NucleoBond® Xtra Midi, Maxi, AX 100, 500, 2 000, and 10 000 Columns | 740415 |
NucleoBond® Xtra Buffer Set I | 1 | for isolation of low-copy plasmids, cosmids, BACs, PACs,and P1 constructs, only applicable with NucleoBond® Xtra kits, sufficient for 10 Xtra Maxi preps or 20 Xtra Midi preps,set includes buffers RES, LYS, NEU, and RNase A | 740417 |