货号: | 740412.50 |
NucleoBond® Xtra Midi / Maxi | Transfection-grade plasmid DNA | |
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Features | ||
The new generation of ultra-fast and high-yield, transfection-grade plasmid DNA purification at Midiprep and Maxiprep scale | ||
• Optimized column design: plasmid Midiprep in approximately 30 min, plasmid Maxiprep in 35 min |
NucleoBond® Xtra Midi | NucleoBond® Xtra Maxi | |||
Technology | Anion-exchange chromatography | Anion-exchange chromatography | ||
Format | Midi gravity flow columns | Maxi gravity flow columns | ||
Lysate clarification | Column filters | Column filters | ||
Sample material | < 200 mL (high copy) < 400 mL (low copy) | < 600 mL (high copy) < 1200 mL (low copy) | ||
Vector size | < 300 kbp | < 300 kbp | ||
Typical yield | 250 µg | 1000 µg | ||
A260/A280 | 1.80–1.95 | 1.80–1.95 | ||
Isopropanol precipitation | Xtra Midi | Xtra Midi Plus | Xtra Maxi | Xtra Maxi Plus |
Preparation time | 70 min/4 preps | 30 min/4 preps | 80 min/4 preps | 35 min/4 preps |
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Applications | ||||
• High-copy plasmids from E. coli • Low-copy plasmids from E. coli* | ||||
* Increased buffer volumes required. See "Ordering information" |