货号: | 740609.250 |
保存条件: | 常温 |
规格: | 250次 |
产品详细描述
PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume | |
• Two applications in one kit, one buffer with optimal performance for both applications |
Technology | Silica-membrane technology |
Format | Mini spin columns |
Sample material | < 400 µL PCR reaction mixture |
Fragment size | 50 bp–approx. 20 kbp |
Typical recovery | 70–95% |
A260/A280 | 1.8–1.9 |
Elution volume | 15–30 µL |
Preparation time | 10 min/6 preps |
Binding capacity | 25 µg |
Applications | |
• Purification of PCR products | |
• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as: - nucleotides, primers - enzymes - mineral oil - PCR additives (e.g., salts, betaine, DMSO) - detergents (e.g., Tween 20, Triton X-100) - dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain) | |
• Support protocol for DNA extraction from polyacrylamide gels | |
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis |