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GGTase Substrate, Fluorogenic
PBS
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| 货号： | M0202 |
| 保存条件： | -20 |
| 规格： | V S L |
T4 DNA Ligase
| M0202L | T4 DNA ligase | 100,000units |
| M0202S | T4 DNA Ligase | 20,000units |
| M0202V | T4 DNA Ligase | 10,000 units |
DescriptionBack to Top
Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates.Highlights
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
Product Source
Purified from E. coli C600 pcl857 pPLc28 lig8.Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| T4 DNA Ligase Reaction Buffer | -20 | 10X |
Advantages and Features
Applications
- Cloning of restriction fragments
- Joining linkers and adapters to blunt-ended DNA
Properties and Usage
Unit Definition
One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer.Concentration:
400,000 cohesive end units/ml and 2,000,000 cohesive end units/ml
Reaction Conditions
1X T4 DNA Ligase Reaction Buffer
Incubate at 16°C
1X T4 DNA Ligase Reaction Buffer:
50 mM Tris-HCl
10 mM MgCl2
1 mM ATP
10 mM DTT
pH 7.5 @ 25°C
Storage Temperature
-20°CStorage Conditions
10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
65°C for 10 min 温馨提示：不可用于临床治疗。
