红色荧光金Fluoro-Ruby（Fluorochrome）

 

Fig.1 Amygdala cells (40X) after  Fluoro-Gold  injection in the PVN.  Antibody is at 1/50,000

Fig.2 High magnification view of Fluoro-Ruby labeled axons, terminals and vascular pericytes as seen in the rat striatum following tracerinjection into the contralateral substantia nigra. ​
 

Tosolini A.P., Mohan R, Morris R., “Targeting the full length of the motor end plates regions in the mouse forelimb increases the uptake of Fluoro-Gold into corresponding spinal cord motor neurons,” Frontiers in Neurology (2013) 1-10.

Tosolini A.P., Mohan R., “Spatial characterization of the motor neuron columns supplying the rat forelimb,” Neuroscience 200 (2012) 19-30.

Bowyer, J.F. and Schmued, L., Fluoro-Ruby labeling prior to an amphetamine neurotoxic insult shows a definitive massive loss of dopaminergic terminals and axons in caudateputamen. Brain Res., 1075, (2006) 236-239.

*Schmued, L.C., “Anti-retrograde and retrograde neuroanatomical tract tracing with fluorescent compounds,” Neuroscience Protocols, 94-050-02 (1994) 1-15.

Van Bockstaele, E.J., Wright, A.M., Cestari, D.M., and Pickel, V.M., “Immunolabeling of retrogradely transported Fluoro-Gold: sensitivity and application to ultrastructural analysis of transmitter-specific mesolimbic circuitry,” Journal of Neuroscience Methods, 55 (1994) 65-78.

*Bowyer, J.F., Gough, B., Broening, H.W., Newport, G.D., and Schmued, L., “Fluoro-Gold and Pentamidine Inhibit the In Vitro and In Vivo Release of Dopamine in the Striatum of Rat,” The Journal of Pharmacology and Experimental Therapeutics, 266 (1993) 1066-1074.

Balercia, G., Chen, S., and Bentivoglio, M., “Electron microscopic analysis of fluorescent neuronal labeling after photoconversion,” Journal of Neuroscience Methods 45 (1992) 87-98. [Link]

*Chang, H.T., Kuo, H., Whittaker, J.A., and Cooper, N.G.F., “Light and electron microscopic analysis of projection neurons retrogradely labeled with Fluoro-Gold notes on the application of antibodies of Fluoro-Gold,” Journal of Neuroscience Methods, 35 (1990) 31-37.

Schmued, L., “Fluoro-Gold and 4-Acetamido-4′-isothiocyanostilbene-2,2’disulfonic Acid: Use of Substituted Stilbenes in Neuroanatomical Studies,” Methods in Neurosciences, 3 (1990) 317-330.

Schmued, L., Kyriakidis, K., & Heimer, L., In vivo anterograde and retrograde axonal transport of the fluorescent rhodamine-dextran-amine, Fluoro-Ruby, within the CNS. Brain Res, 526 (1990) 127-134.

Schmued, L.C., Kyriakidis, K., Fallon, J.H., and Ribak, C.E., “Neurons containing retrogradely transported Fluoro-Gold exhibit a variety of iysosomal profiles: a combined brightfield, fluorescence and electron microscopic study,” Journal of Neurocytology, 18 (1989) 333-343.

*Pieribone, V.A., Aston-Jones, G., “The iontophoretic application of Flouro-Gold for the study of the afferents to deep brain nuclei,” Brain Research 475 (1988) 259-271. [Link]

Reep, R.L., Baccala, M.J., Booth, M.P., and Goodwin, G.S., “Combined retrograde and anterograde tracing of neuronal connections: Fluoro-Gold and autoradiography,” Journal of Neuroscience Methods, 23 (1988) 1-5.

*Schmued, L.C. and Fallon, J.H., “Fluoro-Gold: a fluorescent retrograde axonal tracer with numerous unique properties,” Brain Research, 377 (1986) 147-154.

Notice: The original and only true Fluoro-Gold (Fluorogold) is produced by Fluorochrome, LLC and marketed by Fluorochrome, LLC and Histo-Chem Inc.Fluoro-Gold (Fluorogold) is an exclusive product of Fluorochrome, LLC. It has been sold by Fluorochrome and widely used since 1985. Other companies are marketing a product they claim is the same as or equivalent to Fluoro-Gold. In fact, the chemical structures of these compounds seem to be different from Fluoro-Gold. Certain physical properties of the compounds may be very different.