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4'-6-DIAMIDINO-
MC3R-CRE-BLA CHO-K1 
LightShift Chemilumi| 货号： | T22500 |
| 数量： | 大量 |
| 英文名： | TEMED |
| 规格： | 100ml |
Product Description
Molecular Formula: C6H16N2
Molecular Weight: 116.2
CAS Number: 110-18-9
Boiling Point: 120-122 °C
Refractive Index: 1.4179 (20 °C)
Density: 0.770 g/ml
Molarity: 6.63 M (based on molecular weight and density)
Synonyms: 1,2-Bis(dimethylamino)ethane, TEMED, TMEDA
This product is suitable for electrophoresis and is tested as a catalyst for polyacrylamide gel electrophoresis (PAGE).
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. Such TEMED generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Polyacrylamide gels are commonly used for the electrophoretic resolution of macromolecules such as nucleic acids and proteins. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.
The potential contribution of TEMED in the presence of elevated background in silver stained SDS PAGE has been studied.4 TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy.5 Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED.7 TEMED has been used in the HPLC of small organic molecules.
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions
This product is miscible in water (1 ml/1 ml, 50%), yielding a clear, colorless to faint yellow solution.
| 货号 | 名称 | 英文名称 | 规格 | 单价 |
| Sigma-T22500 | 四甲基乙二胺 | TEMED | 100ml | 196 |
| Sigma-T22500 | 四甲基乙二胺 | TEMED | 500ml | 询价 |
