货号: | H-1000 |
保存条件: | -4°保存 |
供应商: | 乐乾科仪 |
数量: | 大量/现货 |
英文名: | Mounting Medium |
保质期: | 1年 |
规格: | 10ml |
Vector 公司提供多种水溶性和脂溶性封片剂,适合于不同底物。此外, Vector 公司还提供多种房防止荧光萃灭的封片剂(染色片可保存 1-2 个月)和带有染色细胞核( DNA )的封片剂(用于 ISH 或衬染 DNA 的实验等)。
货号: H-1000
包装: 10ml/ 瓶
价格:电议
1. Vector H-1000, Mounting Medium, 400 张 /10ml 防荧光萃灭效果尤好,长期保存。 FITC,Texas Red,Rhodamine,AMCN 等。 2.Vector H-1200 ,Mounting Medium with DAPI, 含 DAPI 400 张 /10ml , 防荧光萃灭;含 DAPI, 可衬染细胞核;用于 ISH 或衬染 DNA 的实验。 PE , FITC 等。 3.Vector H-1300 , 含 PI 400 张 /10ml , 防荧光萃灭;含 PI ,可衬染细胞核;用于 ISH 或衬染 DNA 的实验。 FITC 等。 4.Vector H-1400, HardSet Mounting Medium, 400 张 /10ml 凝固性;防荧光萃灭;适于油镜观察。 FITC,Texas Red,Rhodamine 等。 5.Vector H-1500 HardSet Mounting Medium with DAPI , 400 张 /10ml 凝固性;防荧光萃灭;含 DAPI ,可衬染细胞核,用于 ISH 或衬染 DNA 的实验;适于油镜观察。 FITC,Texas Red, 含 DAPI Rhodamine 等。 6.Vector H-5000 ,Permanent Mounting Medium ,240 张 /60ml 非水溶性,无毒、无味;清晰;和底物 BCIP/NBT 联用,可将结晶减至最小。长期保存。 HRP 和 AP 的底物。 7.Vector H-5501, AQ Aqueous Mounting Medium ,240 张 /60ml 水溶性,适于反应产物为酒精或其它有机溶剂溶性的底物。 大多数 HRP 和 AP 的底物如 AEC , DAB, BCIP/NBT, Vector SG. VECTASHIELD® Mounting Media are unsurpassed in preventing photobleaching. The different formulations of VECTASHIELD® Mounting Media all offer the same outstanding anti-fade and anti-photobleaching properties. They are all compatible with fluorescein, Texas Red®, AMCA, DyLight® dyes, Alexa Fluor® dyes, fluorescent nuclear stains, fluorescent proteins, fluorescent tracers, histochemical stains, and most fluorochromes. Features of VECTASHIELD® Mounting Media: - Inhibit photobleaching of most fluorochromes
- Available in non-hardening or hardening formulations
- Available with or without DAPI or propidium iodide
- No warming necessary
- Slides can be viewed after prolonged storage
- Continues to inhibit photobleaching after prolonged storage of mounted slides
- Easy to use
- Optically clear
- Ideal refractive index
The original VECTASHIELD® Mounting Medium is a glycerol-based, aqueous mountant that does not solidify but remains a viscous liquid on the slide. After mounting, coverslipped slides will not readily dry out and can be reviewed for weeks afterwards without sealing. For prolonged storage, coverslips can be permanently sealed around the perimeter with nail polish. Mounted slides should be stored at 4 ºC. VECTASHIELD® Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (located in product information box) to determine if VECTASHIELD® will be compatible in your system. Please note: VECTASHIELD® Mounting Medium may not be compatible with all enzymatic substrates or fluorescent proteins and test sections would be advisable when using anything other than commonly used fluorochromes. The refractive index for VECTASHIELD® Mounting Medium is 1.44. 1R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182. VECTASHIELD® Mounting Medium Antifade Comparison Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD® mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer’s published results. |
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温馨提示:不可用于临床治疗。
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