货号: | M8042 |
数量: | 2368 |
保存条件: | 2-8℃ |
规格: | M8042-500ML |
MSDS
低限量 Eagle 培养基
Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture (Sigma)
货号与规格 | 价格 (CNY) |
---|---|
M8042-500ML | 527.67 |
M8042-6X500ML | 2,679.30 |
M8042-24X500ML | 9,231.30 |
产品描述
General description
This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. See formulation for details. Often used in cytogenetic analysis when supplemented with FBS.
Linkage
Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.
Reconstitution
Supplement with 0.292 g/L L-glutamine
Application
Minimum Essential Medium Eagle is used for primary osteoblast cultures[3]and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.[2]
Biochem/physiol Actions
MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO3, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.[1]
性质
Related Categories | Classical Media, Culture, General Stem Cell Biology,Minimum Essential Medium Eagle (MEM), Stem Cell Differentiation, Stem Cell Expansion, 干细胞生物学, 细胞培养, 细胞生物学, 经典培养基和盐 Less... |
Quality Level | PREMIUM |
sterility | sterile-filtered |
form | liquid |
impurities | endotoxin, tested |
suitability | suitable for cell culture |
components | NaHCO3: yes L-glutamine: no phenol red: yes HEPES: no sodium pyruvate: yes |
storage temp. | 2-8°C |