货号: | C0498 |
生长状态: | 详见说明书 |
年限: | 详见说明书 |
运输方式: | 详见说明书 |
器官来源: | 详见说明书 |
是否是肿瘤细胞: | 详见说明书 |
细胞形态: | 详见说明书 |
免疫类型: | 详见说明书 |
物种来源: | human |
相关疾病: | 详见说明书 |
组织来源: | 详见说明书 |
品系: | 详见说明书 |
ATCC Number: | 详见说明书 |
细胞类型: | B cell precursor leukemia |
肿瘤类型: | 详见说明书 |
供应商: | 详见说明书 |
数量: | 详见说明书 |
DSMZ no.:ACC 508
Species:human (Homo sapiens)
Cell type:B cell precursor leukemia
Origin:established from the bone marrow of a 32-year-old woman with acute lymphoblastic leukemia (ALL L2) in first relapse
Biosafety level:1
Permissions and restrictions: A
DSMZ Cell Culture Data:
Morphology: single, relatively small, round cells growing in suspension
Medium: 90% alpha-MEM + 10% h.i. FBS
Subculture: split saturated culture 1:3 to 1:4 every 3-4 days; seed out at ca. 0.5 x 106 cells/ml; maintain at about 0.5-1.0 x 106 cells/ml
Incubation: at 37 °C with 5% CO2
Doubling time: ca. 50 hours
Harvest: maximal density of about 4.0 x 106 cells/ml
Storage: frozen with 70% medium, 20% FBS, 10% DMSO
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