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Anti-Human CD99 PE 2
AsPC-1细胞【通过STR
人霍奇金淋巴瘤；Hs 6
GCLC试剂盒
THOP1试剂盒
Trx试剂盒| 货号： | HZ0478 |
| 供应商： | 上海沪震生物科技有限公司 |
| 数量： | 大量 |
| 英文名： | pCMVLacI |
| 保质期： | 1年 |
| 保存条件： | -20摄氏度保存 |
| 规格： | 盒 |
pCMVLacI产品信息
订购信息
| 货号 | 载体名称 | 出品公司 | 载体用途 | 价格 | 到货周期 |
| HZ0478 | pCMVLacI | LacSwith II哺乳动物诱导表达系统 | 1000 | 现货 |
产品参数
Mammalian Selection：Hygromycin
载体描述
Improved System
The improved LacSwitch II inducible mammalian expression system consists of 3 vectors: pOPI3CAT vector from the original LacSwitch system and two new vectors, pCMVLacI and pOPRSVI/MCS, that dramatically improve the system’s performance and versatility. Lac repressor protein, produced from the pCMVLacI vector, blocks transcription by binding the Lac repressor protein to a specific DNA sequence (the operator) in the pOPI3CAT and pOPRSVI/MCS vectors. IPTG, which has no adverse effect in eukaryotic cells, decreases the binding affinity of the Lac repressor protein to the operator sequences, triggering transcription and expression of the inserted gene. The LacSwitch II system allows induction of the gene of interest within 4-8 hours.
Tight Repression
The new pCMVLacI vector replaces the p3’SS vector from the original system. The pCMVLacI vector provides greater repression of the gene of interest due to the increased strength of the CMV promoter. High expression of the lacI gene provides 2- to 10-fold tighter repression of the gene of interest than the original p3’SS vector. A nuclear localization sequence (NLS) directs expression of Lac repressor to the nucleus.
Directional Insertion of the Gene of Interest
To facilitate directional cloning, the multiple cloning site from pBluescript? II SK was inserted into pOPRSVI. The resulting pOPRSVI/MCS vector permits directional insertion of the gene of interest, decreasing the time required for screening. The presence of T3 and T7 promoters on either end of the MCS in the pOPRSVI/MCS vector allows easy sequencing and verification of positive inserts using T3 and T7 primers.
Removal of the RSV Promoter
The pOPI3CAT operator vector is included in the LacSwitch II system for replacing the RSV-LTR promoter with a promoter of interest. This vector contains three operators in the intron and unique restriction sites for removal of the RSV promoter.
载体序列
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