肌酸激酶检测试剂盒

 产品名称 :

分类名称 :酶活性测定,血液尿液化学产品介绍 :

测定方法     比色法（340nm）定量测定肌酸激酶活性。

产品说明    肌酸激酶（CK），也被称作肌氨酸***酸激酶（CPK）是一种主要在骨骼肌﹑平滑肌以及大脑组织中表达的酶。它主要由两个亚基组成，即脑型亚基（B）和肌肉型亚基（M）。且有三种同功酶：CK-MM﹑CK-BB和CK-MB。肌酸激酶能够催化肌氨酸转化为***酸肌酸，并伴随有ATP的消耗和ADP的产生，同时也可催化其逆反应。肌酸激酶通常能在急性胸痛和肾衰竭的病人体内检测到。体内高浓度的肌酸激酶常意味着肌肉损伤，且常见于受伤﹑横纹肌溶解﹑心肌梗塞﹑肌炎﹑心肌炎﹑恶性高烧﹑抗精神病药恶性综合征等疾病。体内低浓度的肌酸激酶则意味着酒精性肝病和风湿性关节炎。简单﹑直接﹑高度自动化的肌酸激酶活性检测手段是十分理想的。本公司产品检测基于酶联反应生成NADPH，在波长340nm下检测，其颜色深浅程度与样品中肌酸激酶活性成正比。

产品特点    测量敏感、准确：采用96孔板测试，肌酸激酶活性检测范围在5-00U/L之间。 操作简便：在室温或37℃环境下，整个过程只需加入工作试剂，并在10分钟和40分钟时读取吸光度即可。高通量：通过96孔板上的高通量自动检测手段，每天可以测试上千个样本。

产品用途    1. 直接检测血清、血浆、尿液和生物制剂中肌酸激酶的活性; 2. 用于药品和药理学研究，检测药物对肌酸激酶活性的影响。

储藏条件    用冰运输，试剂和标准品在-20℃下保存。

DESCRIPTION  CREATINE KINASE (CK), also known as creatine phosphokinase (CPK), is an enzyme (EC 2.7.3.2) expressed predominantly in skeletal muscle, smooth muscle and the brain. The CK enzyme consists of two subunits, which can be either B (brain type) or M (muscle type), and hence three different isoenzymes: CK-MM, CK-BB and CK-MB. CK catalyzes the conversion of creatine to phosphocreatine, consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP) and the reverse reaction. CK is often determined routinely in emergency patients with chest pain and acute renal failure. Elevation of CK is an indication of damage to muscle and has been associated with injury, rhabdomyolysis, myocardial infarction, myositis, myocarditis, malignant hyperthermia and neuroleptic malignant syndrome, etc. Lower levels can be an indication of alcoholic liver disease and rheumatoid arthritis. Simple, direct and automation-ready procedures for measuring CK activity are very desirable. Our Creatine Kinase Assay Kit is based on enzyme coupled reactions. The produced NADPH is measured at 340 nm and is proportionate to the CK activity in the sample.

APPLICATIONS  Direct Assays: CK in serum, plasma and other biological samples. Pharmacology: effects of drugs on CK activity.

KEY FEATURES  Sensitive and accurate. Detection range: 5 to 300 U/L creatine kinase in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 10 min and 40 min at room temperature or 37°C. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

STORAGE CONDITIONS  This kit is shipped on dry ice. Store all reagents at -20°C. Shelf life of at least 6 months.