Disease:Esophageal carcinoma Origin:Established from the adenocarcinoma of the lower esophagus (Barretts metaplasia), equivalent to OE33 Species:Homo sapiens Tissue:Esophagus Morphology:Epithelial Properties:Adherent Cytogenic data:express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice Patient:Female, Caucasian, 73 years of age Medium:RPMI1640 + 10% FBS Subculture:1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility:Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ:EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells References for JROECL33 cells:1. Zhang, Q., Zhao, X. H., and Wang, Z. J. Flavones and flavonols exert cytotoxic effects on a human oesophageal adenocarcinoma cell line (OE33) by causing G2/M arrest and inducing apoptosis. Food Chem Toxicol, 46: 2042-2053, 2008. 2. Beales, I. L. and Ogunwobi, O. O. Leptin synergistically enhances the anti-apoptotic and growth-promoting effects of acid in OE33 oesophageal adenocarcinoma cells in culture. Mol Cell Endocrinol, 274: 60-68, 2007. 3. Cheong, E., Ivory, K., Doleman, J., Parker, M. L., Rhodes, M., and Johnson, I. T. Synthetic and naturally occurring COX-2 inhibitors suppress proliferation in a human oesophageal adenocarcinoma cell line (OE33) by inducing apoptosis and cell cycle arrest. Carcinogenesis, 25: 1945-1952, 2004.