货号: | EC1101-1 |
样本: | 50 μl |
应用: | 血清、血浆、细胞培养上清液、尿液、唾液、牛奶 |
检测方法: | ELISA |
检测限: | 0.03 ng/ml |
供应商: | AssayPro |
数量: | 大量 |
规格: | 96 wells |
补体C1q酶联免疫试剂盒
Introduction
Complement component C1q is the recognition subunit of C1 complex of the classical pathway of complement activation. C1q is a 460-kDa protein with the overall shape of a bouquet of flowers, comprising six heterotrimeric collagen-like triple helices (1-2). The globular heads of the C1q bind to the Fc-fragment of IgM or IgG on the surface of a pathogen, playing an important role in host defense and apoptotic cell clearance. It is a functional ligand for leukocyte-associated Ig-like receptor 1 restricting immune cell differentiation and activation (3). C1q prevents toxicity induced by oligomeric forms of amyloid-β (4). Failure to efficiently clear apoptotic cells in the absence of C1q is associated with lupus-like autoimmunity (5).
Principle of the Assay The AssayMax Human Complement C1q ELISA kit is designed for detection of C1Q in human plasma, serum, saliva, urine, milk, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C1q in less than 4 hours. A polyclonal antibody specific for C1q has been pre-coated onto a microplate. C1q in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C1q, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.