货号: | EL3020-1 |
样本: | 25 μl |
应用: | 牛奶 |
检测方法: | ELISA |
检测限: | 0.09 μg/ml |
供应商: | AssayPro |
数量: | 大量 |
规格: | 96 wells |
人溶菌酶LYZ酶联免疫试剂盒
Lysozyme is one of the anti-microbial agents found in human milk and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. Lysozyme has 130 amino acids, and its natural substrate is the bacterial cell wall peptidoglycan. Since it is synthesized by granulocytes and macrophages, lysozyme can act as a useful marker for myelomonocytic cells (1, 2). Increased levels of lysozyme in urine and serum are diagnostic indicators for acute monocytic leukemia and acute myelomonocytic leukemia (3). Elevated lysozyme levels were found in synovial fluids of the inflammatory arthritides and osteoarthritis (4). Human lysozyme gene mutations cause hereditary systemic amyloidosis (5, 6). The extracellular clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species (7). Salivary lysozyme, a marker for oral infection and hyperglycemia, might display a significant relationship with hypertension, an early stage of cardiovascular disease (8).
Principle of the Assay
The AssayMax Human Lysozyme ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of lysozyme in human milk. This assay employs a quantitative competitive enzyme immunoassay technique that measures lysozyme in less than 3 hours. A polyclonal antibody specific for lysozyme has been pre-coated onto a 96-well microplate with removable strips. Lysozyme in standards and samples is competed with a biotinylated lysozyme sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured