ibidi 细胞定位格子培养皿u-Dish 35 mm, high glass bottom Grid-50/81148
货号 | 产品名称 | 规格(个/盒) |
81148 | μ-Dish 35 mm,高壁,玻璃底,50微米格子培养皿 | 30 |
81168 | μ-Dish 35 mm,高壁,玻璃底,500微米格子培养皿 | 30 |
80156 | μ-Dish 35 mm,低壁,ibiTreat底部处理,500微米格子培养皿 | 60 |
80151 | μ-Dish 35 mm,低壁,无包被,500微米格子培养皿 | 60 |
81166 | μ-Dish 35 mm,高壁,ibiTreat底部处理,500微米格子培养皿 | 60 |
81161 | μ-Dish 35 mm,高壁,无包被,500微米格子培养皿 | 60 |
直径35mm玻璃底培养皿,底部带有50um网格
用于细胞和细胞簇的重找回
便于进行固定区域的细胞计数
玻璃底可用于TIRF和单细胞应用
应用
重新定位细胞和细胞簇,如转染细胞的克隆挑选
对某一划定区域的细胞计数,如计算转染效率
为细胞运动提供一个参考结构
独特的单个细胞处理,如显微注射
技术特征
底部50μm网格
字母和数字标识的4 x 400 个方格(A-U; 1-20)
网格和细胞在一个焦面
底部为D 263 M肖特玻璃,厚度为170 μm +/- 10 μm
盖子有锁扣设计,可以减少蒸发
产品规格
Grid repeat distance | 50 μm | Coating area using 400 μl | 4.1 cm2 |
Ø μ-Dish | 35 mm | Ø observation area | 21 mm |
Volume | 2 ml | Height with / without lid | 14/12 mm |
Growth area | 3.5 cm2 | Bottom | Glass coverslip No. 1.5, selected quality, 170 μm +/- 10 μm |
Layout Grid-50
ibidi Standard Bottom vs. ibidi Glass Bottom
| ibidi Standard Bottom | ibidi Glass Bottom |
Optical properties | ||
Refractive index (nD 589 nm) | 1.52 | 1.52 |
Abbe number | 56 | 55 |
Thickness | #1.5 (180 μm) | #1.5 (170 μm) |
Material | Microscopy plastic | D 263M Schott borosilicate glass |
Autofluorescence | Low | Low |
Transmission | Very high (even ultraviolet) | High (ultraviolet restrictions) |
Birefringence (DIC) | Low (DIC compatible*) | Low (DIC compatible*) |
Other aspects | ||
Surface modifications | ibiTreat – tissue culture treated Uncoated – hydrophobic | Only pure glass |
Protein coatings | Possible | Possible |
Gas permeable | Yes | No |
Material flexibility | High | Low |
Breakable | No | Yes |
Recommended for | All kinds of fluorescence microscopy | TIRF and single photon |