Preparation : | Produced in BALB/c mice using highly pure (>98%) recombinant human TNF-alpha as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography. |
Catalog #: | 500-M26 |
Source : | Mice |
Formulation : | A sterile filtered antibody solution was lyophilized from PBS. |
Stability : | The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. Frozen aliquots are stable for at least six weeks at 2-8oC or for at least six months at -20oC. Avoid repeated freeze-thaw cycles. |
Reconstitution: | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml. |
Applications : | |
Selected References : | Selected List of References Citing This PeproTech Product |
Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of hTNF-α (0.5 ng/ml), a concentration of < 0.1="" μg/ml="" of="" this="" antibody="" is=""> |
Elisa | In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of recombinant human TNF-α when used with PeproTechs biotinylated antigen affinity purified anti-human TNF-α (500-P31ABt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml. |
Western Blot | To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions. |
Country of Origin : | USA |
|
|
| Size B | 500μg | $150 | | |
|
|
|
| Special Sizes: Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request. |
| Usage:For Research Use Only. Not for use in diagnostic or therapeutic procedures. |