货号: | DMSSY510 |
供应商: | 上海笃玛生物科技有限公司 |
数量: | 现货 |
保存条件: | 电询 |
规格: | 100T |
Labeling of Autophagic Vacuoles with monodansylcadaverine (MDC)The autofluorescent agent MDC (Sigma) was recently introduced as a specific autophagolysosome marker to analyze the autophagic process [33]. U251 cells were treated with Res 150 μM for 3–24 h. Autophagic vacuoles were labeled with MDC by incubating cells with 0.05 mM MDC in RPMI1640 at 37°C for 10 min at various times after Res treatment. After incubation, cells were washed three times with PBS and immediately analyzed with a fluorescence microscope (Nikon Eclipse TE 300, Japan) equipped with a filter system (V-2A excitation filter:380/420 nm, barrier filter:450 nm) [34]. Images were captured with a CCD camera and imported into Adobe Photoshop.