H1N1 ( A/California/04/2009 ) NA / Neuraminidase Protein (Active)

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H1N1 ( A/California/04 /2009 ) NA / Neuraminidase Protein
 
 
 
 
 
 
 
H1N1 NA / Neuraminidase Protein Price Inquiry ( Available Sizes )
 
 
 
200μg:  询价;
 
≥1mg 大包装:  询价
 
 
 
H1N1 NA / Neuraminidase蛋白产品信息
 
 
 
 
别名:
 
 
NA
 
 
 
 
表达方式:
 
 
A DNA sequence encoding the mature form of influenza A virus ( A/California/04/2009 ( H1N1 ) ) neuraminidase ( ACP41107.1 ) ( His 36 -Lys 469 ) was fused with a polyhistidine tag at the N-terminus
 
 
 
 
种属:
 
 
Influenza A Virus H1N1
 
 
 
 
表达宿主: 
 
 
Baculovirus
 
 
 
 
 
H1N1 NA / Neuraminidase蛋白质控指标
 
 
 
 
纯度:
 
 
> 88 % as determined by SDS-PAGE
 
 
SDS-PAGE:  
H1N1 NA protein
 
 
 
 
生物活性:
 
 
Measured by its ability to cleave a fluorogenic substrate, 2’-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid The specific activity is 800-1200 pmoles/min/µg
 
 
 
 
内毒素:
 
Please contact us for more information.
 
 
 
稳定性:
 
Samples are stable for up to twelve months from date of receipt at -70℃
 
 
 
预测N端:
 
 
His
 
 
 
 
分子量:
 
The recombinant influenza H1N1 virus neuraminidase ( A/California/04/2009 ( H1N1 ) ) comprises 450 amino acids with the predicted molecular mass of 50 kDa. The apparent molecular mass of the recombinant protein is approximately 55 kDa in SDS-PAGE under reducing conditions
 
 
 
缓冲液:
 
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4 , 10% gly 
 
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
 
Please contact us for any concerns or special requirements.
 
 
 
 
 
 
H1N1 NA / Neuraminidase蛋白使用指南
 
 
 
 
储存方法:
 
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
 
 
 
 
 
Reconstitution:
 
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
 
 
 
 
H1N1 NA / Neuraminidase蛋白相关产品和研究主题
 
 
Related Areas:
 
Enzyme>>Carbohydrate Metabolism Enzymes>>H1N1 neuraminidase/H1N1 NA
 
Immunology>>Innate Immunity>>Lysosomal Enzyme>>H1N1 neuraminidase/H1N1 NA
 
Virus>>Influenza Virus>>H1N1 neuraminidase/H1N1 NA
 
Proteins:
 
 
 
 
Products
 
Source (CLICK for detailed Info. and Price)
 
Molecule
 
Description
 
Cat No
 
 
 
Protein
 
Influenza Neuraminidase (NA) H1N1, A/California/04/2009
 
NA
 
Fc Tag
 
11058-V01H
 
 
 
Protein
 
Influenza Neuraminidase (NA) H1N1, A/California/04/2009
 
NA
 
His Tag
 
11058-V07H
 
 
 
Protein
 
Influenza Neuraminidase (NA) H1N1, A/California/04/2009
 
NA
 
His Tag
 
11058-V07B
 
 
 
Protein
 
Influenza Neuraminidase (NA) H1N1, A/California/04/2009 
 
NA
 
His Tag
 
11058-V07HC
 
 
 
Protein
 
Influenza Neuraminidase (NA) H1N1, A/California/04/2009 
 
NA
 
 -
 
11058-VNAHC
 
 
 
Protein
 
Influenza Neuraminidase (NA) H1N1, A/California/04/2009 
 
NA
 
 -
 
11058-VNAHC1
 
 
 
 
Antibodies:
 
 
 
 
Products
 
Antigen Source  (CLICK for detailed Info. and Price)
 
Antigen Molecule
 
Application
 
Cat No
 
 
 
MAb
 
Influenza Antibody to H1N1,  Mouse Anti H1N1 MAb
 
NA
 
WB, ELISA
 
11058-MM04
 
 
 
 
 
Related Influenza Virus Research Tools
 
 
 
Influenza Virus Research Tools
 
Influenza Neuraminidase & Antibody
 
Influenza Neuraminidase (NA) Protein
 
H1N1 Neuraminidase
 
A/California/04/2009
 
 
 
H1N1 NA / Neuraminidase蛋白背景综述
 
Neuraminidase ( NA ) and hemagglutinin ( HA ) are major membrane glycoproteins found on the surface of influenza virus. NA, also called sialidases, specifically catalyze the hydrolysis removal of terminal sialic acid residues from viral and cellular glycoconjugates. It is known that HA binds to the sialic acid-containing receptors on the surface of host cells during initial infection, and at the end of an infectious cycle, NA cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. NA thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell. NA is a single-pass type II membrane protein which exists as a homotetramer, and the transmembrane domain is involved in lipid raft association during intracellular transport. NA is suggested to play a role in the determination of host range restriction on replication and virulence. Nine subtypes of NA have been identified, and subtypes N1 and N2 have been positively linked to epidemics in man.
 
参考文献
 
 
 
Barman, S. and Nayak, D.P. 2000, J. Virol. 74: 6538-6545
 
Colman, P.M. et al., 1983, Nature. 303: 41-44
 
Suzuki, T. et al., 2005, J. Virol. 79: 11705-11715
 
von, Itzstein, M. 2007, Nat. Rev. Drug. Discov. 6: 967-974