载体系统
杂交系统:
哺乳动物双杂交系统
Promega:2500
Vectors:pACT,pBIND
Contorl plasmid:pACT-MyoD,pBIND-Id
Reporter plasmid:pG5 luc
Stratagene:3500
Vectors:pCMV-BD,pCMV-AD
Contorl plasmid:pBD-p53,pBD-NF−κB,pAD-SV40T,pAD-TRAF
Reporter plasmid:pFR-luc
酵母双杂交系统
Clontech:2800
Vectors:pGBKT7,pGADT7,
Positive contorl:pCL1,pGBKT7-53,pGADT7-T
Negative control:pGBKT7-Lam
Strain:AH109
Vector:pACT2 AD
Stratagene:1500
Vectors:pSos,pMyr
Control plasmid:pSos MAFB,
Strain:
酵母单杂交系统
Clontech:800
Vector:pHis2
酵母三杂交系统
Clontech:1500
Vector:pBridge
Strains:Y2H Gold Yeast strain,Y187
诱导系统:
蜕皮***诱导表达系统
Invitrogen:1200
Vectors:pIND,pVgRxR
LacSwith II哺乳动物诱导表达系统
Stratagene:1800
Vectors:pOPRSVI,pOPI3CAT,pCMVLacI
GeneSwith 哺乳动物诱导系统
Invitrogen:1200
Vectors:pSwitch,pGene v5-His B (BamHI-EcoRI)
四环素诱导系统
Invitrogen:2500
Vectors:pcDNA4/TO/Myc-His A,pcDNA4/TO/Myc-His B,pcDNA4/TO/Myc-His C,
Contorl plasmid:pcDNA4/TO/Myc-His/LacZ
Regulatory plasmid:pcDNA6/TR
Clontech:3600
Vectors:pTet-On,pTet-Off,pTRE2,pTRE2 hygro(mcs区含有luciferase片段),pTK-hyg
pRevTet-On,pRevTet-Off,pRevTRE
pTet on advanced,pTRE Tight,pTRE Tight Luciferase(一套2400)
pCMV-Tet3G,pTRE3G,pTRE3G-Luc(一套3000)
Tale ToolBox kit (TALEN and TALETF):8000
Vectors:pNI v2,pNG v2,pNN v2,pHD v2,pTALETF v2 (NN),pTALETF v2 (NG)
pTALETF v2 (NI),pTALETF v2 (HD),pTALEN v2 (NI),pTALEN v2 (NG)
pTALEN v2 (NN),pTALEN v2 (HD)
腺病毒系统:
Stratagene:3500
Vectors:pShuttle-CMV,pShuttle,pAdTrack-CMV,pAdTrack,pAdEasy-1
Strains:BJ5183,AdEasy-1
Invitrogen:1000
Vector:pAd/BLOCK-iT-DEST RNAi Gateway Vector,
Microbix:1600
Vector:pDC315,pBHGloxdelE13cre
腺相关病毒系统:
Stratagene:3000
Vector:pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP,
pCMV-MCS
逆病毒系统:
RNAi
Clontech:1800
Vectors:RNAi-Ready pSIREN-RetroQ,
RNAi-Ready pSIREN-RetroQ-DsRed-Express
RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide),
Ambion:1000 x2
Vectors:pSilencer5.1-U6-Retro,pSilencer5.1-H1-Retro
基因表达载体
Clontech:800x2+800x3
Vectors:pRetroX-IRES-ZsGreen1,pRetroX-IRES-DsRed Express,
pLNCX2,pQCXIH,pMSCVpuro,
Addgene:800 x2+800
Vectors:pSuper retro puro, pSuper retro GFP-neo,pBabe puro
逆病毒包装载体
Addgene:1000
Vectors:pCMV-Gag-pol,pCMV-VSV-G,
Imgenex:1000
Vectors:pCL Ampho
Stratagene:1000
Vectors:pVPack-GP,pCMV VSV G,
慢病毒系统:
RNAi载体
Addgene:800+800+800 x5
Vectors:pSicoR,pSicoR pGK puro,pLentilox 3.7,pLKO.1 puro,pLKO.3G,
pPRIME-TET-GFP-FF3,pPRIME-TREX-GFP-FF3
Clontech:1500
Vectors:pLVX-shRNA1,pLVX-shRNA2
SBI:1000
Vectors:pSIH1-H1-CoGFP
Open Biosystems:800x2+800x2
Vectors:pGIPZ empty,pGIPZ control,pTRIPZ empty,pTRIPZ control
基因表达载体
Clontech:
Vectors:pLVX-DsRed-Monomer-N1(1000),pLVX-AcGFP1-N1(1000),
pLVX-IRES-ZsGreen1(1000),pLVX-IRES-neo(1000),pLVX-IRES-td Tomato(1000),pLVX-IRES-puro(1000),pLVX-puro(1000),
pLVX-Tight-Puro,pLVX-Tet-On-Advanced(一套2000)
pLVX-TRE 3G,pLVX-Tet 3G(一套2400)
Invitrogen:1000 x3
Vector:pLenti4 TO/V5 DEST,pLenti6 V5 DEST,pLenti6-UbC-V5-DEST
SBI:1000 x2
Vectors:pCDH-CMV-MCS-EF1-copGFP,pCDH-CMV-MCS-EF1-GFP+Puro (pCD513B-1)
Addgene:800x3
Vector:pWPXL,FUGW,pLVTH
慢病毒包装载体
Invitrogen:1000x3+800x2
Vectors:pLp1,pLp2,pLp VSV G
Strain:Stbl3,Stbl4
Cell biolabs:800
Vectors:pCgpV
Clontech:800
Vector:pVSV G
Addgene
Second generation:1000
Vectors:pCMV-dR8.91,pCMV-VSV-G
Second generation:1000
Vectors:pMD2.G,pSPAX2
Third generation:1200
Vectors:pMDLg pRRE,pRSV-rev,pCMV-VSV-G
iPS慢病毒载体
Cell biolabs:1000
Vectors:pLentG-KOSM
Addgene:1000
Vectors:FUW-M2rtTA,TetO-FUW-OSKM
SBI (FIV):一套1800
Vectors:pCDF1-MCS2-EF1-copGFP,pFP93
建永生化细胞系慢病毒载体
Addgene:1000 x3
Vector:pLOX-CWBmi1,pLOX-TERT-iresTK,pLOX-Ttag-iresTK
PiggyBac转座子系统
Vectors:HA-mPB(1000),PiggyBac 5’LTR (pMD18T vector),(800)
PiggyBac 3’LTR (pMD18T vector)(800)
SBI:一套2400
Vectors:PiggyBac Dual promoter (PB513B-1),Super PiggyBac Transposase (PB200PA-1)
信号通路报告系统:
Clontech:800/个
Vectors:pGAS-TA-Luc,pSTAT3-TA-Luc,pISRE-TA-Luc,pTA-Luc,
pIκB-EGFP,pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-TA-Luc
pCRE-Luc,pGRE-Luc,pHSE-Luc
Stratagene 800/个
Vectors:p53-Luc,pAP-1-Luc,pNF-κB-Luc,pSRE-Luc,
Promega 800/个
Vectors:pGL4.10,pGL4.26,pGL4.29,pGL4.30,pGL4.75,pGL4.19
Millipore 800/个
Vectors:TOPFlash,FOPFlash,SuperTopFlash
RNAi系统:
Ambion:800+800x6
Vectors:pSilencer1.0,pSilencer 2.1-U6 hygro,pSilencer 3.1-H1 hygro,pSilencer 3.1-H1 neo,
pSilencer 4.1-CMV neo,pSilencer 4.1-CMV puro
Reporter plasmid:pMIR-REPORT Luciferase
Promega:800+800
Reproter plasmid:psiCHECK-2,pmir GLO
Oligoengine:800
Vector:pSuper-puro
Other:800
Vector:pGenesil-1
Clontech:1000
Vector:pmR mCherry
SBI:1000
Vector:pmiRZip anti-microRNA
酵母表达系统:
酿酒酵母
Invitrogen:1800
Vectors:pYES2,pYES6/CT,pYES2/CT/α-factor,
Strain:INVSc1
毕赤酵母
Invitrogen:800/个
Vectors:pGAPZα A,pPICZα A,pPICZα B,pPICZα C,pPIC9K,pPIC3.5K,pPICZ A,pPICZ B,pPICZ C,
Strains:X33,KM71,KM71H,GS115,SMD1163,SMD1168,
Invitrogen:2400
Vector:pPink HC,pPink LC
Strain:Pichiapink-1,Pichiapink-4
Gateway系统:
Invitrogen:800 /个
Vectors:pENTR 1A,pENTR3C,pDONR221,pcDNA6.2-GWEmGFP-miR negative,
pDONR Zeo
Regulatory plasmid:pLenti6/TR
Strain:DB3.1
表面展示系统:
酵母表面展示
Invitrogen:1000
Vector:pYD1
Strain:EBY100
哺乳动物表面展示
Invitrogen:800
Vector:pDisplay
噬菌体表面展示:800/个
Vector:pCantab 5e
Strain:HB2151,TG1
重组蛋白乳汁表达系统:
Invitrogen:1000
Vector:pBC1
绝缘子检测系统:
Invivogen:1000
Vector:pWHERE
果蝇细胞表达系统:
Invitrogen:1200
Vector:pAc5.1 V5-His B,pCo hygro
哺乳动物表达系统:
Invitrogen
Vectors:pcDNA3.1(neo)+ (800),pcDNA3.1(neo)- (800),pcDNA3.1(hyg)+ (800),pcDNA3.1(zeo)+ (800),pcDNA3.1/GS(800),pcDNA3.1(-)/myc-His A(800),pcDNA4/HisMax B(800),pcDNA6-Myc/His B(800),pSecTag2 A(800),pVAX1(800),pBudCE4.1(800),pTracer CMV2(800), pCEP4(800),pUB6/V5 His A(800),pUB6/V5 His B(800),pUB6/V5 His C(800),pUB6/V5 His lacZ(800),
pSFV1(800),pEF1/myc-His B(800), pcDNA3.1(+)/Myc HisB(800),pEF6/Myc-HisC(800),
Stratagen
Vector:pCMV-Tag 2 B(800),pCMV-Tag 5B(800),pNTAP-B(800),pBK-CMV(800),pIRES-hrGFP-1a(800),pSG5(800)
Promega:800 x3
Vectors:pCI-neo,pCI,pSI
Clontech 800一个
Vectors: pCMV-myc,pCMV-HA,pIRES,pIRES neo,
pIRES hyg3,pIRES neo3,pIRES puro3(EcoRI-NotI之间连入Mus musculus CD1d1 antigen),pIRES2-EGFP,pIRES puro2,
Sigma
Vectors:pFLAG CMV2(800)
Invivogen
Vectors:pVitro2-neo-mcs(800)
Addgene
Vectors:pCAGGS(800)
昆虫细胞表达系统:
Invitrogen:800 x12
Vectors:pFastBac1,pFastBac1-Gus,pFastBacHT A,pFastBacHT B,pFastBacHT C,pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A,pBlueBacHis2 B,
pBlueBacHis2 C,pVL1392,pVL1393,
Strain:DH10Bac(800)
Vectors:pIZT/V5-His (800),pMIB v5-His B (800)
Cell:sf9,High Five
MultiBac Expression System:3000
Vectors:pFBDM,pUCDM,pBADZ-His6Cre,
Strains:DH10MultiBac,BW23473
Cell:
荧光蛋白报告系统:
Clontech 800/个
Vectors: pDsRed2-N1,pDsRed2-C1, pDsRED-Monomer-N1,pmCherry-N1,pmCherry-C1, pEYFP-N,pEYFP-C1,pEGFP-N1,pEGFP-C1,pEGFP-N3,pEGFP-1,pAcGFP1-1,pECFP-N1,pECFP-C1,pDsRED2-ER,pDsRED2-Mito,pDsRED2-Nuc,pDsRED2-Peroxi,pEYFP-Golgi,pEYFP-Mem,pEYFP-ER,pEYFP actin,pEYFP tub,pEGFP-Actin,pECFP-ER,pECFP-Mito,pLEGFP-N1,pLEGFP-C1, pDsRed2-1,pEGFP-C3,pPAmCherry-Tubulin,pZsYellow-N1,pAmCyan-N1
FRET assay:800 x2
Vectors:Dkeima fluorescent protein,mAmetrine,
启动子增强子报告系统:
Promega:800 x7
Vectors:pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter,
pRL-TK,pRL-CMV,pRL-SV40
NEB:800
Vectors:pGluc-basic
原核表达系统:
Novagen 800/个
Vectors:pET-11a(+),pET-17b(+),pET-19b(+),pET-20b(+),pET-21a(+),pET-22b(+),pET-26b(+),pET-27b(+),pET-28a(+),pET-28b(+),pET-29a(+),pET-30a(+),
pET-32a(+),pET-39b(+),pET-40b(+),pET-42a(+), pET49b(+),pET50b(+),pETduet-1,pACYCDuet-1,pRSFDuet-1,pCDFDuet-1
Strains:BL21(50),BL21(DE3) ,BL21(DE3)plysS,Rosetta(DE3) ,Rosetta-gami 2(DE3)pLysS,Rosetta-gami B(DE3)pLysS,BLR(DE3) ,BL21(DE3)gold,Rosetta Blue(DE3) ,Rosetta(DE3)plysS,Rosetta-gami(DE3) ,Origami(DE3) ,OrigamiB(DE3) ,
Takara:1000
Vectors:pCold I,pCold TF
NEB:1500
Vectors:pMAL-c2x,pMAL-c4x,pMAL-p4x
Biosee:800/个
Vector:pLLP-OmpA,pLLP-STII,pMBP-P,pMBP-C,pET-GST,pET-Trx,
pET-His,pTrc-CKS,pET-DsbA
Stratagene:800
Strain:BL21 codonplus(DE3)RIPL,
Invitrogen :1000
Vector:pRSET A
Strain:BL21(AI)
Qiagen:1000
Vector:pQE30
Strain:M15
NEB:800x5
Vectors:pTWIN1,pTYB11,pMXB10,pTXB1
Strain:ER2566
Amersham:800/个
Vectors:pGEX-4T-1,pGEX-5X-2,pGEX-6P-1,pGEX-2TK
Invitrogen:800/个
Vectors:pBADHis A,pBADHis B,pBADHis C,
Lucigen:800
Strain:C43(DE3)
Other:1000
Vector:pBV220,pBluescript II SK(+)
Takara
分子伴侣系统:800/个
Vector:pG-KJE8,pGro7,pKJE7,pG-Tf2,pTf16,
基因载体:
Invitrogen
Vector:pOG44(800)
Invivogen
Vectors:pORF-lacZ(800),pORF-HSV1tk(800)
克隆载体:
Promega:800/个
Vectors:pUC18,pUC19,pBR322,pSP64,pSP73
柯斯载体:
Stratagene:800
Vector:SuperCos1
同源重组载体:1000
Vector:pSIM5,pSIM6
细菌基因敲除系统:800/个
Vector:pKD3,pKD4,pKD20,pKD46,PCP20(30度),
Strain:BW25113
克隆菌株:
Strains:DH5α(800),JM110(800),TOP10(800),XL2-Blue MRF’ (800),Mach1 T1(800),
OmniMAX2 T1Phage-Resistant Cells(800),XL10 Gold(800),EZ10(800)
DH10B(800),SURE(800),
基因:
Genes: human TERT(1000),Taq(1000),Pfu (pET16b(+): NdeI-BamHI 2325bp,1200),KOD (pET21b+,,1800),T4 DNA ligase (pET15b+,优化的T4 DNA连接酶,1800),hOct4,hSox2,hKlf4,hC-Myc,mOct4,mSox2,mKlf4,mC-Myc,human CD40L,human DHFR,human Hspa1a,human IgG1 Fc,mouse GM-CSF,mouse IFN-gamma,mouse IL4,mouse IL18,Cre,
pET28a-TEV,klentaq(T vector),Vaccinia viru Topoisomerase I(T vector),sfGFP,iCre,Lysozyme(pPICZα A),GST-3C,mutant Taq(pET28,扩增血液DNA),M-MLV,SUMO(pET28a),mouse RI(pET28)T4 PNK(pET28)
1000/个
慢病毒载体
SBI :
pCDH-EF1-MCS-T2A-Puro
pCDH-EF1-MCS-T2A-Puro
Addgene :
pLVTHM 1000
pLOX-CW-CRE 1000
酵母表达载体:
pP-αSUMO3 1000
SUMO protease 2 1000
真核表达载体:
pBI CMV1 1000
BL21gold(DE3) plysS 800
pE-SUMO 800
真核表达乳酸菌:
pMG36e 800
MG1363 800