LPS诱导的急性炎症模型实验方案:1) 动物适应性生长1星期后随机分组, 每只一般为10只动物;2) ***做阳性要,灌胃给药后半小时,尾静脉注射0.1mg/kg LPS;3) LPS刺激1小时或/和2小时后取血, 检测TNF-a等细胞因子;4) 细胞因子检测请参照ELISA试剂盒操作手册。
Brief protocol of LPS induced TNF-a release1) Divide these experiment rats intosix groups, every group has 10 mice at random. 2) LPS 0.1mg/kg body weight is injected intravenously through the tail vein 30 min after the administration of cpd.3) 60min or 120 min later, blood are collected by retro-orbital puncture by using 5ml tube coated with heparin saline.4) Collect plasma and froze it in -70℃ until TNF-α is analyzed by ELISA