ConA 诱导的急性炎症模型实验方案:1) 无菌生理盐水配置ConA溶液 (1mg/ml), 冰上放置;2) 给药后一小时尾静脉注射ConA溶液;3) ConA注射1个半小时后取血,肝素抗凝;4) 离心后血浆检测IL-2等细胞因子。
Brief protocol of ConA induced IL-2 release assay1) 1 mg/ml of Con A was suspended in sterile saline. Store the Con A solution in ice.2) One hour following compound administration (PO), mice are given an IV injection of Con A (10mg/kg)3) Ninety minutes after the Con A injection, blood was collected in a Heparin sodium containing tube.4) Blood samples were spun at 3,000 rpm at 4℃ for 10 minutes. Plasma were stored in -80℃ freezer until further analysis.